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首页> 外文会议>Conference on Advanced Biomedical and Clinical Diagnostic Systems >Design, fabrication, and characterization of polymeric BioMEMS for the detection of Feline Immunodeficiency Virus (FIV)
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Design, fabrication, and characterization of polymeric BioMEMS for the detection of Feline Immunodeficiency Virus (FIV)

机译：用于检测猫直接免疫缺陷病毒（FIV）的聚合物生物米的设计，制备和表征

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This project comprises the development of a novel polymeric BioMEMS device capable of rapidly detecting FIV in a minimally invasive manner. FIV severely inhibits the infected feline from mounting an immune response, and causes susceptibility to other types of diseases. Vaccines against FIV do exist, but have some strong limitations to their effectiveness; so early detection is the best method to combat the spread of the disease. Current testing methods look for antibodies to the FIV protein p24 in feline blood using established Enzyme Linked ImmunoSorbent Assay (ELISA) protocols. The focus of this research is to design and construct a device that can detect antibodies to p24 in a salivary sample by non-intrusive electrochemical means. The device is constructed upon a silicon substrate with gold microelectrodes coated with polypyrrole (Ppy), an electrically conducting and biocompatible polymer. In the current phase of the research, the Ppy deposition process has been optimized with regards to film thickness, uniformity and conductivity. Microfluidic channels have been fabricated using SU-8, an epoxy based polymer that enables the test sample and other solutions to pass freely through the device. The Ppy will be coated with anti-FIV p24 antibodies that can capture FIV p24 antigens present in a salivary sample. Future research will involve the analysis of Ppy/antibody interaction and its effect on functionality. The capture of such antigens will interfere with a reduction-oxidation (redox) reaction in a subsequently added ionic solution. This interference will change the characteristic resistance of the solution yielding a qualitative test for the presence of the viral antigens in the sample and hence determining the occurrence of infection.
机译：该项目包括开发一种能够以微创方式快速检测FIV的新型聚合物生物体装置。 FIV严重抑制受感染的素线安装免疫应答，并导致对其他类型的疾病的易感性。反对FIV的疫苗确实存在，但对其有效性有一些强烈的局限性;因此，早期检测是打击疾病传播的最佳方法。目前的测试方法使用已建立的酶联免疫吸附测定（ELISA）方案寻找猫血液中FIV蛋白P24的抗体。本研究的重点是通过非侵入式电化学手段设计和构建可以在唾液样品中检测到P24抗体的装置。该装置在具有涂有聚吡咯（PPY）的金微电极的硅衬底上构造，导电和生物相容性聚合物。在研究的当前阶段，PPY沉积过程已经通过关于膜厚度，均匀性和导电性而优化。通过SU-8制造微流体通道，是一种基于环氧树脂的聚合物，使得测试样品和其他溶液能够自由通过该装置通过。 PPY将涂覆有抗FIV P24抗体，其可以捕获唾液样品中存在的FIV P24抗原。未来的研究将涉及分析PPY /抗体相互作用及其对功能的影响。这种抗原的捕获将干扰随后添加的离子溶液中的还原 - 氧化（氧化还原）反应。这种干扰将改变溶液的特征性，从而产生样品中病毒抗原的定性测试，因此确定感染的发生。

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