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首页> 外文会议>International Gluten Workshop >Using Epitope Tagging to Explore the Trafficking, Location and Functional Properties of Wheat Gluten Proteins
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Using Epitope Tagging to Explore the Trafficking, Location and Functional Properties of Wheat Gluten Proteins

机译：使用表位标记来探索小麦麸质蛋白的贩运，位置和功能性质

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A series of transgenic plants are being generated expressing epitope-tagged forms of LMW subunit, HMW subunit and y-gliadin proteins in order to study their trafficking and impact on functional properties. The use of three different tags (c-myc, FLAGand HA) will allow the trafficking of the three proteins to be studied simultaneously in the same cells. The cereal grain is a highly complex structure with variation in composition between and within tissues. In particular, the starchy endosperm exhibits clear gradients in composition, with the sub-aleurone cells being rich in protein with little starch and the central cells being rich in starch with little protein. This variation can be exploited to isolate flour fractions comprising between about 11%and 40% protein (Kent, 1966). Furthermore, the composition of gluten proteins may also vary across the endosperm, with evidence for this coming from the differences in the expression patterns conferred by the promoters of low molecular weight (LMW) andhigh molecular weight (HMW) subunit genes (Lamacchia et al, 2001; Stoger et al, 2001). Support for this also comes from analysis of barley grain, using pearling fractions (Millet et al, 1991; She wry et al, 1996) and immunolocalization (Davies et al, 1993). This heterogeneity, together with the high polymorphism exhibited by gluten proteins, makes it difficult to follow the pathways of synthesis and deposition of individual gluten proteins in the cells of the developing endosperm and to relate this to their role in gluten structure and grain functional properties.
机译：一系列转基因植物正在表达表位标记形式的LMW亚基，HMW亚基和Y-Gliadin蛋白，以研究其贩运和对功能性质的影响。使用三种不同的标签（C-MYC，Flagand HA）将允许在同一细胞中同时进行三种蛋白质的贩运。谷粒是一种高度复杂的结构，具有组织之间的组成变化。特别地，淀粉胚乳在组合物中表现出透明梯度，亚α0酮细胞具有富含淀粉的蛋白质，中央细胞富含淀粉，少量蛋白质。可以利用这种变异以分离粉状馏分，其包含约11％和40％蛋白质（肯特，1966）。此外，麸质蛋白的组成也可以在胚乳上变化，具有来自低分子量（LMW）和高分子量（HMW）亚基基因的启动子（Lamacchia等）的促进剂赋予的表达模式的差异的证据（Lamacchia等， 2001; Stoger等，2001）。支持这一点也来自大麦谷物的分析，使用珠林分数（Millet等，1991; Shry等，1996）和免疫循环化（Davies等，1993）。这种异质性与麸质蛋白表现出的高多态性，使得在显影胚乳的细胞中难以遵循单个麸质蛋白的合成和沉积的途径，并将其与其在麸质结构和晶粒功能特性中的作用相关。

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