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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Antimicrobial activity of chitosan against Fusarium oxysporum f. sp. tracheiphilum
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Antimicrobial activity of chitosan against Fusarium oxysporum f. sp. tracheiphilum

机译：壳聚糖对镰刀菌F的抗菌活性F。 sp。 trachiphilum.

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Fusarium wilt, caused by Fusarium oxysporum f.sp. tracheiphilum, is an important cowpea disease in the Brazilian Northeast. Control of this disease is difficult, since the use of chemical methods causes environmental damage and induction of resistance in pathogens. An alternative for the treatment of Fusarium wilt is the biological control, which may occur by the induction of resistance in the plant through the application of chitosan in soil. Chitosan, a cationic amino polysaccharide, essentially composed of β-1,4 D-glucosamine (GlcNAc) linked to N-acetyl-D-glucosamine residues, is naturally present in the cell wall of certain fungi, and can also be obtained by chitin deacetylation from the exoskeleton of crustaceans, insects and arthropods. In food technology chitosan is readily seen due to its several functional properties and can be used as an antimicrobial agent. The aim of this study was to investigate the antifungal activity, in vitro, of chitosan, from Cunninghamella elegans UCP 542, against three pathogens Fusarium oxysporum f. sp. tracheiphilum. Chitosan was extracted from C. elegans biomass by alkali-acid treatment. Chemical characterization were effected by infrared spectroscopy (Deacetilation degree) and viscosity (Molecular weight). The effectiveness of chitosan isolated from C. elegans in inhibiting the growth of Fusarium oxysporum f. sp. tracheiphilum was evaluated. Chitosan solutions at concentrations ranging from 10.0 to 0.025 mg/mL was prepared in acetic acid 0.5% (v/v), pH adjusted by 5.5. The antifungal activity was assessed by determining the minimum inhibitory and fungicidal concentration using broth dilution method in Saboura medium. Chitosan was replaced with sterile distilled water and 0.5% acetic acid in the positive control. Microbial growth was observed in all positive control. Also, the viability of the F. oxysporum was confirmed by verifying their growth in Sabouraud agar without adding chitosan. The chitosan showed the degree of deacetylation and the viscosimetric molecular weight respectively of 85% and 2.72 _x 10~4 g/mol. Chitosan showed minimum inhibitory concentration and minimum fungicide concentration for F. oxysporum assayed in 0.5 mg/mL and 4.0 mg/mL, respectively. The exact mechanism of the antimicrobial action of chitosan is still unknown, but different mechanisms have been proposed, which consider its chemical and structural properties. The results obtained in this study demonstrate the antifungal potential of chitosan against phytopathogenic fungi.
机译：Fusarium Wilt，由Fusarium oxysporum F.sp引起。 Trachiphilum，是巴西东北的重要豇豆疾病。这种疾病的控制很困难，因为使用化学方法导致环境损伤和诱导病原体的抗性。治疗镰刀菌枯萎的替代方案是生物控制，通过在土壤中诱导植物抗性诱导抗性可能发生。壳聚糖，一种阳离子氨基多糖，基本上由与N-乙酰-D-葡糖胺残基连接的β-1,4 D-葡糖胺（GlcNAc）组成，自然存在于某些真菌的细胞壁中，也可以通过甲酸肽获得从甲壳类动物，昆虫和节肢动物的外骨骼中脱乙酰化。在食品技术中，壳聚糖由于其几种功能性，并且可以用作抗微生物剂。本研究的目的是探讨从苏宁伊育耳奇UCP 542的乌宁乳杆菌，胰蛋白杆菌UCP 542的抗真菌活性。 sp。 trachiphilum。通过碱酸处理从C.杆状杆菌生物量提取壳聚糖。通过红外光谱（脱象）和粘度（分子量）实现化学表征。壳聚糖中胶囊中的抑制抑制牡蛎F的生长。 sp。龟背岩被评估。在乙酸0.0-025mg / ml的浓度下壳聚糖溶液以乙酸0.5％（v / v），pH调节5.5。通过在Saboura培养基中使用肉汤稀释方法确定最小抑制和杀真菌浓度来评估抗真菌活性。壳聚糖被无菌蒸馏水和0.5％乙酸在阳性对照中取代。在所有阳性对照中观察到微生物生长。而且，通过在萨比琼脂的生长而不添加壳聚糖来证实F. oxysporum的活力。壳聚糖显示脱乙酰化程度和分别为85％和2.72℃10〜4g / mol的粘接性分子量。壳聚糖分别显示出在0.5mg / ml和4.0mg / ml的0.5mg / mL和4.0mg / ml中测定的F. oxysporum的最小抑制浓度和最小杀菌剂浓度。壳聚糖抗微生物作用的确切机制仍然是未知的，但提出了不同的机制，这考虑了其化学和结构性。本研究中获得的结果证明了壳聚糖对植物疗法真菌的抗真菌潜力。

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