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Bioprospection of microorganisms for lipase production using an industrial waste as carbon source

机译:使用工业废物作为碳源的脂肪酶生产微生物的生物痉挛

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The aim of this study was the bioprospection of microorganisms isolated from the effluent of the cassava flour for lipase production. Samples of bacteria, yeasts and filamentous fungi were isolated from this effluent. The experiments were carried out in Erlenmeyers flasks in the presence of "manipueira", olive oil and ammonium sulphate, at 150 rpm and 28 °C, during 8 days. The lipase production increased with the cultivation time for 45 % of the microorganisms investigated. Some microbial samples showed different peaks of lipolytic activity. The maximum lipase production reached 10.1 IU/L at pH 5.6 and the maximum productivity was 3.95 IU/L.d at pH 3.9. More research needs to be stimulated on bioprospection of microorganisms for enzyme production with the reuse of industrial wastes, avoiding environmental impact and reducing costs for biotechnological processes.
机译:该研究的目的是从木薯粉的流出物中分离的微生物的生物痉挛用于脂肪酶生产。从该流出物中分离出细菌,酵母和丝状真菌的样本。在8天内,在“人脂素”,橄榄油和硫酸铵,在150rpm和28℃下,在Erlenmeyers烧瓶中进行实验。脂肪酶生产随着45%的微生物进行培养时间而增加。一些微生物样品显示出不同的脂溶性活性峰。 pH 5.6的最大脂肪酶生产达到10.1 IU / L,pH 3.9的最高生产率为3.95 IU / L.D。需要刺激更多的研究,以促进工业废物的再利用,避免环境影响和降低生物技术过程的成本,促进酶生产的生物痉挛。

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