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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Presence of Arcobacter spp. contamination in fresh lettuces for human consumption
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Presence of Arcobacter spp. contamination in fresh lettuces for human consumption

机译：Accobacter SPP的存在。用于人类消费的新鲜莴苣中的污染

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Arcobacters are considered emerging foodborne pathogens frequently isolated from animal food products and various types of water. However, there is no data on the presence of Arcobacter spp. in fresh vegetables and given that the consumption of salads has increased, it could be interesting to monitoring its microbiological contamination. Therefore the objective of this research was to study the presence of Arcobacter spp. in fresh lettuces. Fifty fresh lettuces purchased from different local shops in Valencia (Spain) between January and July of 2009 were analyzed. The assay was performed simultaneously by PCR and cultural methods. Arcobacter sp. was detected in 10 of the 50 samples by PCR, but just in one of them the detection was possible without enrichment. A. butzleri was the only detected species by multiplex PCR. Seven samples were found to be positive also by culture. Nineteen isolates were obtained, being all of them identified as A. butzleri. To our knowledge, this is the first report of Arcobacter spp. detection in fresh vegetables such as lettuces. Although these foods are generally considered safe, given the large quantities consumed and the fact that further cooking is absent, they could be a potential public health risk.
机译：Arcobacters被认为是从动物食品和各种类型的水分中分离出来的出现新兴的食源性病原体。但是，没有关于Arcobacter SPP存在的数据。在新鲜蔬菜中，鉴于沙拉的消费增加，监测其微生物污染可能是有趣的。因此，本研究的目的是研究SPP Arcobacter的存在。在新鲜的莴苣中。分析了2009年1月至7月在瓦伦西亚（西班牙）的不同当地商店购买的五十鲜良品莴苣。通过PCR和培养方法同时进行测定。 Arcobacter sp。通过PCR检测到50个样品中的10个中，但在其中一个没有富集的检测中。 A. Butzleri是Multiplex PCR的唯一检测到的物种。发现七个样品也被培养为阳性。获得了19分离物，将所有物质鉴定为A. Butzleri。为了我们的知识，这是第一个Accobacter SPP的报告。在新鲜蔬菜中检测如莴苣。虽然这些食物通常被认为是安全的，但鉴于所消耗的大量和进一步烹饪的事实，它们可能是潜在的公共卫生风险。

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