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首页> 外文期刊>International Journal of Food Microbiology >Study of Arcobacter spp. contamination in fresh lettuces detected by different cultural and molecular methods.
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Study of Arcobacter spp. contamination in fresh lettuces detected by different cultural and molecular methods.

机译：Arcobacter spp的研究。通过不同的文化和分子方法检测到的新鲜生菜中的污染物。

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Arcobacters are considered potential emerging food and waterborne pathogens. However, there is no data on the presence of Arcobacter spp. in fresh vegetables. Therefore the objective of this research was to study the presence of Arcobacter spp. in fresh lettuces. Fifty fresh lettuces purchased from different local shops in Valencia (Spain) were analyzed. The assay was performed simultaneously by cultural and molecular methods. Isolates were identified by real-time, multiplex PCR and restriction fragment length polymorphism analysis of PCR-amplified DNA fragment (PCR-RFLP). Finally, all the isolates were genotyped using the randomly amplified polymorphic DNA (RAPD-PCR) method. Arcobacter sp. was detected in 10 of the 50 samples (20%) by real-time PCR, being A. butzleri the unique detected species by mPCR. The detection levels obtained by conventional PCR (7 samples/50, 14%) were slightly lower. These seven samples were found to be positive also by culture isolation. All 19 obtained isolates were identified as A. butzleri by multiplex PCR and PCR-RFLP. Great genetic heterogeneity among the isolates was observed by RAPD-PCR profiling. To our knowledge, this is the first study in which Arcobacter spp. is detected in fresh vegetables such as lettuces. Although these foods are generally considered safe, given the large quantities consumed and the fact that further cooking is absent, lettuce could be a source of Arcobacters of public health concern.
机译：芽孢杆菌被认为是潜在的新兴食品和水生病原体。但是，没有关于 Arcobacter spp的数据。在新鲜蔬菜中。因此，本研究的目的是研究 Arcobacter spp的存在。在新鲜的生菜中。分析了从西班牙巴伦西亚不同地方商店购买的五十种新鲜生菜。通过文化和分子方法同时进行测定。通过实时，多重PCR和PCR扩增的DNA片段（PCR-RFLP）的限制性片段长度多态性分析，鉴定出分离物。最后，使用随机扩增多态性DNA（RAPD-PCR）方法对所有分离株进行基因分型。 Arcobacter sp。通过实时PCR在50个样品中的10个（20％）中检测到A。通过mPCR检测到的独特菌种。通过常规PCR获得的检测水平（7个样品/ 50，14％）略低。通过培养分离也发现这七个样品也是阳性的。获得的所有19种分离物均被鉴定为i。多重PCR和PCR-RFLP筛选布茨列里氏菌。通过RAPD-PCR分析发现分离株之间存在很大的遗传异质性。据我们所知，这是 Arcobacter spp的第一项研究。在生菜等新鲜蔬菜中被检出。尽管通常认为这些食品是安全的，但考虑到食用量大且缺乏进一步烹饪的事实，生菜可能是引起公众健康的弧菌的来源。

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