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Isolation and screening of soil microorganisms for membrane-active antimicrobial metabolites

机译：膜活性抗菌代谢物土壤微生物的分离和筛选

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摘要

The focus of this study was antimicrobial membrane-activity of microorganisms isolated from soils of Iran. In this work soil samples were collected from desert and farming zones of Northern and Central of Iran. A total number of 75 species consist of 36 bacteria, 28 fungi and 11 actinomycetes were isolated from the soil samples. In the primary screening that was performed to evaluate antimicrobial activity, isolated microorganisms were analyzed in terms of their general inhibition effects to indicator strains E. coli, C. albicans, and S. cervisiae. It has been found that 23 isolates including 4 bacteria, 13 fungi and 6 actinomycetes, were effective against test microorganisms. In the secondary screening to determine membrane-active metabolites producing microorganisms, isolates which had an inhibitory effect against test microorganisms, were analyzed for membrane activity using a Rapid Chromatic Detection method. Based on color and fluorescence changes that are easily identified by the naked eye and fluorescent microscope, 3 species consist of 1 fungus and 2 actinomycetes had membrane-activity effect and were stored for the sake of further study and identification.
机译：这项研究的重点是从伊朗的土壤中分离的微生物的抗菌膜活动。在这项工作中的土壤样品是从沙漠和北部与伊朗中部的养殖区收集。的75种的总数包括36个细菌，真菌28和11米的放线菌是从土壤样品中分离。在已执行以评价抗微生物活性的初级筛选，分离的微生物中的其一般抑制作用到指示器条款进行分析菌株大肠杆菌，白色念珠菌和酿酒酵母。已经发现，23株包括4个细菌，13种真菌和6米的放线菌，反对试验微生物有效。在二次筛选，以确定膜的活性代谢物的微生物，其具有针对试验微生物的抑制作用，分离株进行了分析使用快速色检测方法膜活性。在此基础上很容易被肉眼和荧光显微镜识别出的颜色和荧光变化的3种，包括1种真菌和放线菌2具有膜 - 活性的影响并储存用于进一步研究和鉴定的缘故。

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