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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Vanadium pentoxide: an oxidative stress agent which disturbs glutathione conjugates metabolization in Saccharomyces cerevisiae UE-ME3
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Vanadium pentoxide: an oxidative stress agent which disturbs glutathione conjugates metabolization in Saccharomyces cerevisiae UE-ME3

机译：五氧化二钒：一种氧化应激剂，其在酿酒酵母中扰乱谷胱甘肽缀合物的酿酒酵母葡萄球菌UE-ME3

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摘要

Under physiological conditions the vanadium can be detected in the oxidation state V (+5), a very toxic specie. In this study we used a wine wild-type Saccharomyces cerevisiae UE-ME_3 to assess how eukaryotic cells respond to V_2O_5. Yeast cells in exponential phase were inoculated into YEPD medium with 2% (w / v) glucose and allowed to grow in the absence or presence of 0.5 to 2.0 mM V_2O_5 during 72 h. The results show that the cellular environment became more oxidizing, cell damages increase and failures in the transport and excretion of amino acids to the vacuole ocurr in yeast cells grown in the presence of vanadium (+5), as can be inferred by the decrease of GSH/GSSG ratio, increase of MDA cytoplasmic level and the sharp decrease of γ-GT activity observed. Although there was an apparent response anti-stress to V_2O_5, reflected in an increase of GR activity in all trials of metal exposure and of GPx in cells exposed to 1.5 and 2.0 mM, this was probably mitigated by the decrease in activity G6PD and consequent availability of NADPH which compromise cell viability.
机译：在生理条件下，可以在氧化态V（+5）中检测钒，这是一个非常有毒的物种。在这项研究中，我们使用了葡萄酒野生型酿酒酵母UE-ME_3来评估真核细胞如何响应V_2O_5。指数相中的酵母细胞接种到具有2％（w / v）葡萄糖的YePD培养基中，并在72h期间在不存在或存在下生长0.5-2.0mM V_2O_5。结果表明，细胞环境变得更加氧化，细胞损坏在钒（+5）存在下生长的酵母细胞中的氨基酸的运输和排泄中的氨基酸的损失和损失增加，可以推断下降GSH / GSSG比率，MDA细胞质水平的增加和观察到γ-GT活性的急剧下降。虽然对V_2O_5的表观反应抗应激抗应力反映在金属暴露的所有试验中GR活性的增加，但在暴露于1.5和2.0mm的细胞中，这可能减少了G6PD的降低和随之可用性而减轻了这一点损害细胞活力的NADPH。

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