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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Investigating candidate factors influencing bovine spongiform encephalopathy brainstem sample quality in a beef abattoir
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Investigating candidate factors influencing bovine spongiform encephalopathy brainstem sample quality in a beef abattoir

机译：调查影响牛屠宰场牛海绵状脑病脑干质量的候选因素

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Obtaining the appropriate sample is critical to ensure bovine spongiform encephalopathy (BSE) test result integrity. For BSE, this is the brainstem at the level of the obex - the area where abnormal prion protein (PrP) is most consistently deposited and fortuitously first detectable. Occasionally suboptimal samples (SO) occur where the obex is absent or unidentifiable, in which case negative results are questionable. The relationship between SO occurrence and factors such as animal age, breed category [dairy, beef breeds native to the British Isles and Continental beef breeds], gender, skinning method (upward or downward) and sampler identity (n=13) was investigated. A stepwise logistic regression model was applied to a dataset containing records of 23,646 animals sampled at the abattoir over a 2-year-period. Details relating to SO occurrence were obtained from laboratory reports. Details relating to the animal's age and date of slaughter, gender and breed were obtained from the Animal Identification and Movement (AIM) database (Department of Agriculture, Food and the Marine, Ireland). Skinning method changed mid study. SO incidence was 0.26%. Results indicate that three individual samplers, bulls and native and continental beef breeds had a significant positive effect on SO occurrence (P<0.05). Age and skinning method were found not to have any significant effect. The results inform a basis for risk profiling animals prior to sampling. The importance of sampler training and motivation is also indicated. It is recommended that samplers perfect their technique by sampling animals younger than the statutory prescribed age prior to taking official samples. Suggestions to elaborate the current study are discussed.
机译：获得适当的样本对于确保牛海绵状脑病（BSE）测试结果完整性至关重要。对于BSE来说，这是obex水平的脑干 - 朊病毒蛋白（PRP）最常一致地沉积和偶然可检测到的区域。偶尔会出口样本（SO）发生在缺乏或无法识别的地方，在这种情况下，负面结果是值得怀疑的。研究了动物年龄，品种类别[乳品，牛肉品种的因素等因素之间的关系研究，性别，剥皮方法（向上或向上或向上或向上）和采样器标识（n = 13）。将逐步逻辑回归模型应用于在2年期间在AbattoR中采样的23,646只动物的记录的数据集。与所以发生的细节是从实验室报告中获得的。与动物年龄和屠宰日期有关的详细信息，从动物识别和运动（AIM）数据库（农业，食品和海洋，爱尔兰）中获取。皮肤方法改变了中期研究。所以发病率为0.26％。结果表明，三个单独的采样器，公牛和本地和欧洲牛肉品种对所发生的显着阳性作用（P <0.05）。发现年龄和皮肤方法没有任何显着影响。结果为在抽样之前提供了风险分析动物的基础。还指出了采样器培训和动机的重要性。建议采样者通过比法定规定年龄更年轻的动物在官方样本之前进行比例。讨论了详细说明目前的研究的建议。

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