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首页> 外文会议>ICME International Conference on Complex Medical Engineering >Differential Proteome of the Striatum from A30P α-Synuclein Transgenic Mouse Model of Parkinson's Disease
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Differential Proteome of the Striatum from A30P α-Synuclein Transgenic Mouse Model of Parkinson's Disease

机译：来自A30Pα-突触核蛋白转基因小鼠帕金森病的晶体形状的差异蛋白质组

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摘要

Parkinson's disease (PD) is a multifactorial, neurodegenerative disease where etiopathogenesis are not fully understood. Mutations in α-Synuclein (α-Syn) were the first genetic defect linked to PD. They are deposited in Lewy bodies (LBs) characteristic for PD. Some experiments had showed that A30P mutant α-Syn have high toxicity than wide-type α-Syn. Here we used A30Pα-Syn transgenic mice model to analysed proteome changes of the striatum 11 months after the birth. Striata were removed and after digesting the proteins we used isotope labelling method to mark different group of peptides. Strong-cation exchange (SCX) liquid chromatography (LC) was integrated with peptide separation as the first dimension of the two-dimensional LC tandem mass spectrometry workflow. In this work, electrospray ionization (ESI) quadrupole time-of-flight (QTOF) mass spectrometer was explored as a means of detecting the Ms/Ms spectrogram. Agilent Spectrum Mill software was used to analysed the results. A total of 660 proteins were quantified. 280 proteins were down-regulated and 77 proteins were up-regulated.
机译：帕金森病（PD）是一种多因素，神经退行性疾病，其未得到完全理解。 α-突触核蛋白（α-SYN）中的突变是与PD相关的第一个遗传缺陷。它们沉积在Lewy体内（LBS）的PD特征。一些实验表明，A30P突变体α-SYN具有高于宽型α-SYN的毒性高。在这里，我们使用A30Pα-SYN转基因小鼠模型分析出生后11个月后纹状体的蛋白质组变化。除去斯特丽特并在消化蛋白质后，我们使用同位素标记方法标记不同的肽。强阳离子交换（SCX）液相色谱（LC）与肽分离集成为二维LC串联质谱工作流程的第一尺寸。在这项工作中，探讨了电喷雾电离（ESI）四极杆飞行时间（QTOF）质谱仪作为检测MS / MS谱图的方法。 Agilent Spectrum Mill软件用于分析结果。总共量化了660个蛋白质。将280个蛋白质下调，77个蛋白质被上调。

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