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首页> 外文会议>International Conference on Medical Physiology >Recent developments in High Resolution Tandem Mass Spectrometry for biomolecules and related compounds
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Recent developments in High Resolution Tandem Mass Spectrometry for biomolecules and related compounds

机译：用于生物分子和相关化合物的高分辨率串联质谱的最新发展

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In recent years several tandem mass spectrometric instruments like triple quadrupole (QqQ), TOF/TOF and hybrid systems like quadrupole/time-of-flight (Q-TOF), ion trap/time-of-flight (IT-TOF), LIT-Orbitrap and many others were introduced, each one offering several critical advantages and allowing the analyst to select the proper solution regarding the particular type of application. Except of sector field analyzers, nowadays hybrid MS can provide sufficiently high resolution values. Since the most efficient and commonly applied separation techniques are liquid and gas chromatography and capillary electrophoresis, the selected instrumentation must be eligible to hyphenate with some of them, and for this purpose there are suitable interfaces like, for instance, atmospheric pressure ionization (ESI and APCI) and photoionization (APPI), electron impact ionization (EI), chemical ionization (CI), fast atom bombardment (FAB) or even plasma atomizers (ICP and MIP), which in principle function as mild or hard ion sources for MS. Nevertheless for high molecular weight analytes like proteins, nucleotides, natural and synthetic polymers, other ionization techniques like for instance matrix-assisted laser desorption/ionization (MALDI) are definitely required since they allow for improved sensitivity in extended mass range. Sophisticated software for optimization of the experimental procedures, manipulating of the large set of data and identification of unknown compounds and structures is required.
机译：近年来，几个串联质谱仪器，如三重四极（QQQ），TOF / TOF和混合系统，如四边形/飞行时间（Q-TOF），离子陷阱/飞行时间（IT-TOF），点燃 - 介绍了许多其他人，每个人都提供了几种关键优势，并允许分析师选择关于特定类型的应用的适当解决方案。除了扇区外分析仪，现在混合动力MS可以提供​​足够高的分辨率值。由于最有效且常用的分离技术是液体和气相色谱和毛细管电泳，所以所选择的仪器必须有资格与其中一些相关的，并且为此目的，存在合适的界面，例如大气压电离（ESI和APCI）和光离子化（APPI），电子碰撞电离（EI），化学离子化（CI），快速原子轰击（FAB）甚至等离子体雾化器（ICP和MIP），其原则上用作MS的温和或硬离子源。然而，对于蛋白质，核苷酸，天然和合成聚合物等高分子量分析物，肯定需要其他电离技术，例如，允许基质辅助激光解吸/电离（MALDI），因为它们允许改善延长质量范围的敏感性。用于优化实验程序的复杂软件，需要操纵大量数据和未知化合物和结构的鉴定。

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