• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Advances in Materials and Manufacturing Processes >A novel scaffold from recombinant spider silk protein in tissue Engineering
【24h】

A novel scaffold from recombinant spider silk protein in tissue Engineering

机译：来自组织工程中重组蜘蛛丝蛋白的一种新型支架

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

As a new biomaterial, recombinant spider silk protein has attracted much attention in tissue engineering. The pNSR-16/BL21(DE3)pLysS strains fermented and produced the recombinant spider silk protein, which was then cast into scaffolds. NIH-3T3 cells were cultivated with extractions of the scaffolds in vitro. The cytotoxicity of scaffolds was analyzed with a MTT assay. The performances of cells adhesion, growth and expression on the scaffolds were observed with SEM, HE staining and immunohistochemistry. Compared with the control, the extract fluid of materials culturing the NIH-3T3 cells was not apparently different. NIH-3T3 cells could adhere and grow on the scaffolds and secret FGF-2. The pNSR-16 recombinant spider silk protein scaffolds has satisfactory cytocompatibility and the scaffolds are ideal scaffold material for tissue engineering.
机译：作为一种新的生物材料，重组蜘蛛丝蛋白在组织工程中引起了很多关注。 PNSR-16 / BL21（DE3）帘布层菌株发酵并制备重组蜘蛛丝蛋白，然后将其铸造成支架。用体外萃取支架培养NiH-3T3细胞。用MTT测定分析支架的细胞毒性。用SEM，HES染色和免疫组化观察到支架上的细胞粘附，生长和表达的性能。与对照相比，培养NIH-3T3细胞的材料的提取液并不明显不同。 NIH-3T3细胞可以粘附并在支架上生长和秘密FGF-2。 PNSR-16重组蜘蛛丝蛋白支架具有令人满意的细胞粘合性，并且支架是用于组织工程的理想支架材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号