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Hydrothermal Pretreatment of Oil Palm Fronds for Increasing Enzymatic Saccharification

机译：用于增加酶糖化的油棕榈叶的水热预处理

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Oil palm fronds are one of the most promising agricultural lignocellulosic residues for bioethanol production and for adding value to chemical feedstocks in Indonesia and South Kalimantan. Currently, hydrothermal pretreatment has attracted a great deal of attention because it only uses chemical-free water, which is a non-toxic, inexpensive, and environmentally friendly method to enhance enzymatic digestibility in the production of fermentable sugars. In this study, oil palm fronds were treated at temperatures ranging from 170 to 210 °C for 30 minutes in a batch autoclave. The effect of the temperature and time of hydrothermal pretreatment on the characteristics of the oil palm fronds and the amount of reducing sugar was expressed as a severity factor (log Ro). Furthermore, after the hydrothermal process the changes in the mass loss, color of substrates, and the pH of filtrate were evaluated. From these results, the mass loss and pH indicated that the optimum severity factor for hydrothermal pretreatment was 4.72. Additionally, the alteration to the oil palm frond structure was confirmed by Fourier Transform Infrared (FTIR) spectroscopy. Finally, 48.8% (w/v) fermentable sugars were obtained from enzymatic hydrolysis using cellulase enzyme at the newly determined maximum severity factor of 4.72.
机译：油棕叶状物是生物乙醇生产中最有前景的农业木质纤维素残留物之一，并在印度尼西亚和南荷马丹的化学原料增加价值。目前，水热预处理引起了大量的关注，因为它仅使用无氧水，这是一种无毒，廉价，环保的方法，以提高发酵糖的生产中的酶消化率。在该研究中，在批量高压釜中在170至210℃的温度下处理油棕叶片30分钟。水热预处理温度和时间对油棕榈叶的特性的影响及还原糖量的效果表示为严重程度因子（LOG RO）。此外，在水热过程后，评价底物的质量损失，颜色和滤液的pH的变化。从这些结果，质量损失和pH值表明水热预处理的最佳严重性因素为4.72。另外，通过傅里叶变换红外（FTIR）光谱证实了对油棕色晶体结构的改变。最后，使用纤维素酶在新确定的最大严重性因子为4.72的最大严重因子中从酶水解中获得48.8％（w / v）可发酵的糖。

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