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首页> 外文会议>International Conference on Energy and Environment Research >Production of immobilized extracellular lipase from Aspergillus niger by solid state fermentation method using palm kernel cake,soybean meal,and coir pith as the substrate
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Production of immobilized extracellular lipase from Aspergillus niger by solid state fermentation method using palm kernel cake,soybean meal,and coir pith as the substrate

机译：使用棕榈仁蛋糕，大豆蛋糕，牛肉蛋糕和髓髓中固态发酵法从曲霉尼日尔的固定化细胞外脂肪酶生产

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摘要

Microbial lipase has shown great potentials as a biocatalyst due to its ability to perform hydrolytic activities at low temperature and pressure conditions.Many researches prove that agro-industrial residues can be an excellent substrate for the production of lipase by solid state fermentation(SSF).This study aimed to produce extracellular lipase from solid state fermentation of filamentous fungi Aspergillus niger by SSF on agro-industrial residues such as palm kernel cake,soybean meal,and coir pith.Fermentation was carried out at room temperature,initial pH of 7,with no stirring or aeration.Produced enzymes were later characterized at several inducer concentration and incubation period.This research obtained lipase with highest activity of 163.33 U/g dss using soybean meal with 9 days of incubation and addition of 4% olive oil.Lipase activity was further investigated by spray drying and immobilization using anion-macroporous resin.Spray dried lipase showed enzyme loading of 53.7%.Immobilized enzyme was analyzed by utilizing it as a biocatalyst for interesterification reaction in non-alcohol route of biodiesel synthesis in batch reactor with 1:12 of reactant palm oil and methyl acetate at 40 °C and 50 hour cycle.Immobilized enzyme has 47.3% biodiesel yield and maintained 57% of its initial activity after four cycle of interesterification.
机译：微生物脂肪酶已经由于其在低的温度和压力conditions.Many研究进行水解活性的能力显示出巨大的潜力作为生物催化剂证明农工业残渣可以是用于通过固态发酵（SSF）的生产脂肪酶的极好底物。本研究旨在通过SSF上农工业残渣如棕榈仁蛋糕，大豆粉，和椰壳pith.Fermentation以产生来自丝状真菌黑曲霉的固态发酵的细胞外脂肪酶，在室温下，为7初始pH值，以进行没有搅拌或aeration.Produced酶后进行了表征在几个导流浓度和温育period.This研究得到163.33 U /克DSS使用大豆粉的最高活性脂肪酶与温育和添加4％的橄榄oil.Lipase活性的是9天通过喷雾干燥，并使用阴离子大孔resin.Spray干燥的脂肪酶固定化进一步研究显示出53.7％.Immo酶负载bilized酶通过利用它在40℃和50小时的时间，在间歇式反应器的生物柴油合成的非醇路线酯交换反应的生物催化剂与反应物的棕榈油和乙酸甲酯1:12分析cycle.Immobilized酶具有47.3％的生物柴油收率和经过四个周期交换的保持其初始活性的57％。

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