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Bioinformatics analysis of the phytoene synthase gene in cabbage (Brassica oleracea var. capitata)

机译:白菜植物合成酶基因的生物信息学分析(甘蓝醛植物。Capitata)

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Phytoene Synthase (PSY) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. capitata PSY (BocPSY) gene sequences were obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocPSY1, BocPSY2 and BocPSY3 genes mapped to chromosomes 2,3 and 9, and contains an open reading frame of 1,248 bp, 1,266 bp and 1,275 bp that encodes a 415, 421, 424 amino acid protein, respectively. Subcellular localization predicted all BocPSY genes were in the chloroplast. The conserved domain of the BocPSY protein is PLN02632. Homology analysis indicates that the levels of identity among BocPSYs were all more than 85%, and the PSY protein is apparently conserved during plant evolution. The findings of the present study provide a molecular basis for the elucidation of PSY gene function in cabbage.
机译:植物合酶(PSY)是类胡萝卜素生物合成中的重要酶。在这里,芸苔Oleracea var。 Capitata Psy(Bocpsy)基因序列是从甘蓝型数据库(Brad)获得的,并进行了生物信息学分析。映射到染色体2,3和9的Bocpsy1,Bocpsy2和Bocpsy3基因,并含有1,248bp,1,266bp和1,275bp的开放阅读框,它们分别编码415,421,424氨基酸蛋白。亚细胞定位预测所有Bocpsy基因在叶绿体中。 Bocpsy蛋白的保守结构域是PLN02632。同源性分析表明,Bocpsys之间的身份水平均超过85%,并且在植物演进过程中,Psy蛋白显然是保守的。本研究的发现为卷心菜阐明了Psy基因功能的分子基础。

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