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首页> 外文会议>International Molecular Medicine Tri-Conference. >A Pilot Study of Expression and Clinical Significance of Stem Cell-Like Markers CD133 or CD24 in Different Subpopulations of Gastric Cancer Circulating Tumor Cells
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A Pilot Study of Expression and Clinical Significance of Stem Cell-Like Markers CD133 or CD24 in Different Subpopulations of Gastric Cancer Circulating Tumor Cells

机译：干细胞样标志物CD133或CD24在胃癌循环肿瘤细胞不同亚群中的表达及临床意义的试验研究

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Background: Cancer stem cells (CSCs), characterized as undifferentiated cells that are responsible for tumor initiation and regeneration, have been an active focus in the field of cancer research. During tumorigenesis, subsets of tumor cells within the primary tumor might acquire features of motility and invasiveness and enter blood vessels. These changes are accompanied by a process called epithelial-mesenchymal transition (EMT), and lead to a more mesenchymal or even more stem cell-like phenotype in circulating tumor cells (CTCs). In this work, a preliminary study was performed on the association between the CD 133+ or CD24+ CSCs and different EMT phenotypes CTCs, and clinicopathological features in patients with gastric cancer (GC). Methods: The CTCs were isolated from peripheral blood of 39 GC patients. The CanPatrol CTC system was applied to enumerate and characterize the phenotypes of CTCs. The first step of this technique was to isolate CTCs via a filter-based method; then, a quantifiable, quadruple-colorimetric RNA in situ hybridization (ISH) method was used to characterize cells according to the expression status of epithelial and mesenchymal biomarkers, CD45, and CD 133 or CD24. Results: Our result showed that CTCs were detected in 32 (82%) of 39 GC blood samples, and five categories of CTCs were defined using EMT biomarkers ranging from exclusively epithelial (E) to intermediate (E > M, E = M, M > E) and exclusively mesenchymal (M). The expression status of CD 133 was examined in 19 samples and CD24 in 20 samples, and 31.6% (6/19) of cases were positive for CD133 expression, and 25% (5/20) of cases were positive for CD24 expression. CD133+ and CD24+ CSCs were more commonly found in epithelial phenotypes (E or E > M), with 75% of CD133+ CSCs and 80% of CD24+ CSCs in epithelial phenotypes respectively. In the CD 133 group, Of the GC patients undergoing distant metastasis, 67% (4/6) of cases was positive for CD 133 expression, and CD 133 expression was significantly associated with distant metastasis (P=0.025); while in the CD24 group, only 20%(1/5) of cases was positive for CD24 expression in the GC patients undergoing distant metastasis, and there was no association of CD24 with distant metastasis (P=0.78) Conclusions: Although the sample size of this study was small, our findings suggested that the CD 133+ CTCs might be a potential predictive marker for the metastatic spread of gastric cancer.
机译：背景：癌症干细胞（CSCs），其特征为肿瘤引发和再生的未分化细胞，是癌症研究领域的积极焦点。在肿瘤发生期间，原发性肿瘤内的肿瘤细胞亚组可能获得动力和侵袭性的特征，并进入血管。这些变化伴随着称为上皮 - 间充质转换（EMT）的过程，并在循环肿瘤细胞（CTC）中导致更密闭或甚至更多的干细胞状表型。在这项工作中，对CD 133+或CD24 + CSC和不同EMT表型CTC之间的关联进行初步研究，以及胃癌（GC）患者的临床病理特征。方法：CTCs与39克GC患者的外周血分离。应用CanpaTrol CTC系统枚举并表征CTC的表型。该技术的第一步是通过基于滤光片的方法分离CTC;然后，使用原位杂交（ISH）方法的可量化的四重比色RNA根据上皮和间充质生物标志物，CD45和CD133或CD24的表达状态表征细胞。结果：我们的结果表明，在32例（82％）的39个GC血液样品中检测到CTC，并使用从专门上皮（E）到中间体（E> M，E = M，M，E = M，M > E）和专门间充质（M）。在19个样品中检测CD 133的表达状态，在20个样品中，31.6％（6/19）的病例对于CD133表达为阳性，25％（5/20）例对于CD24表达为阳性。在上皮表型（E或E> M）中更常见的CD133 +和CD24 + CSC，分别具有75％的CD133 + CSC和80％的上皮表型CD24 + CSC。在CD133组中，在接受远处转移的GC患者的患者中，67％（4/6）病例对于CD 133表达为阳性，CD 133表达与远处转移显着相关（P = 0.025）;在CD24组中，在接受远处转移的GC患者中，仅20％（1/5）的病例对于CD24表达是阳性的，并且没有CD24与远处转移的关联（P = 0.78）结论：虽然样品大小本研究较小，我们的研究结果表明CD 133+ CTC可能是胃癌转移扩散的潜在预测标志。

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