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首页> 外文会议>Institute of Electrical and Electronics Engineers International Conference on Nano/Micro Engineered and Molecular Systems >3D Microstructures Array Single-cell-based DEP Chip for Studying Apoptosis of U937 A431 Cells
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3D Microstructures Array Single-cell-based DEP Chip for Studying Apoptosis of U937 A431 Cells

机译：3D微结构阵列基于单细胞的DEP芯片，用于研究U937＆A431细胞凋亡

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This study presents the design, simulation, fabrication and preliminary results of a cell trap device for detecting the early stage of apoptosis, PS (phosph-atidylserine) exposure, under single-cell resolution. The structure of biochip is consisted of ITO top electrode, PDMS flow chamber, bottom electrode array and SU-8 3D microstructure array. In order to achieve single-cell resolution, we utilize the excimer laser micromachining technique to simultaneously fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array, which design not only constructs a nonuniform electric field for trapping cells in the flow chamber by dielectrophoresis (DEP), but also enhances the positioning and immobilization of trapped cells. In addition, the optimal electrode width based on the simulation results is 30 mum for a cell size in the range of 15~20 mum such as U937 (Human histiocytic lymphoma) and A431 (Human epidermoid carcinoma) cancer cells. By using this DEP chip, we can study the physiological change of apoptosis in one single cell instead of whole cell population. As the preliminary results, 25 mum latex beads can be successfully trapped with single-bead resolution in a short time. Furthermore, the time- and dose-dependent manners for A431/As2O3 and U937/Taol are also confirmed by agarose gel electrophoresis and flow cytometry but not on-chip monitoring. We are still working on real-time and on-chip study for development of a high-throughput drug screening with single-cell level
机译：本研究介绍了在单细胞分辨率下检测细胞捕集装置的设计，模拟，制造和初步结果，用于检测细胞凋亡，PS（磷酸丝氨酸丝氨酸）暴露的早期阶段。 Biochip的结构包括ITO顶电极，PDMS流量室，底部电极阵列和SU-8 3D微结构阵列。为了实现单电池分辨率，我们利用准分子激光微加工技术同时制造棋盘型底部电极阵列和碗型3D微结构阵列，该阵列不仅构建了用于捕获流量中的细胞的非均匀电场腔室通过电泳（DEP），但也增强了捕获细胞的定位和固定。另外，基于模拟结果的最佳电极宽度为30毫米，用于诸如U937（人组织细胞淋巴瘤）和A431（人表皮癌）癌细胞的15〜20毫米的电池尺寸。通过使用该DEP芯片，我们可以研究一种单细胞中凋亡的生理变化而不是全细胞群。作为初步结果，在短时间内，25米胶乳珠子可以以单珠分辨率成功捕获。此外，A431 / AS2O3和U937 / TAOL的时间和剂量依赖的方式也通过琼脂糖凝胶电泳和流式细胞术进行证实，但没有片上监测。我们仍在正实时和片上研究，用于开发具有单细胞水平的高通量药物筛选

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