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首页> 外文会议>Multimodal biomedical imaging VI >Particle velocity measurements with macroscopic fluorescence imaging in lymph tissue mimicking microfluidic phantoms
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Particle velocity measurements with macroscopic fluorescence imaging in lymph tissue mimicking microfluidic phantoms

机译：模仿微流控幻影的淋巴组织中宏观荧光成像的粒子速度测量。

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Ultrasound poroelastography can quantify structural and mechanical properties of tissues such as stiffness, compressibility, and fluid flow rate. This novel ultrasound technique is being explored to detect tissue changes associated with lymphatic disease. We have constructed a macroscopic fluorescence imaging system to validate ultrasonic fluid flow measurements and to provide high resolution imaging of microfluidic phantoms. The optical imaging system is composed of a white light source, excitation and emission filters, and a camera with a zoom lens. The field of view can be adjusted from 100 mm x 75 mm to 10 mm x 7.5 mm. The microfluidic device is made of polydimethylsiloxane (PDMS) and has 9 channels, each 40 μm deep with widths ranging from 30 μm to 200 urn. A syringe pump was used to propel water containing 15 μm diameter fluorescent microspheres through the microchannels, with flow rates ranging from 0.5 μl/min to 10 ul/min. Video was captured at a rate of 25 frames/sec. The velocity of the microspheres in the microchannels was calculated using an algorithm that tracked the movement of the fluorescent microspheres. The imaging system was able to measure particle velocities ranging from 0.2 mm/sec to 10 mm/sec. The range of flow velocities of interest in lymph vessels is between 1 mm/sec to 10 mm/sec; therefore our imaging system is sufficient to measure particle velocity in phantoms modeling lymphatic flow.
机译：超声多孔弹性成像可以量化组织的结构和机械特性，例如硬度，可压缩性和流体流速。人们正在探索这种新颖的超声技术，以检测与淋巴疾病相关的组织变化。我们已经构建了一个宏观荧光成像系统，以验证超声流体流量测量并提供微流体体模的高分辨率成像。光学成像系统由白光源，激发和发射滤光片以及带变焦镜头的照相机组成。视场可以从100毫米x 75毫米调整到10毫米x 7.5毫米。该微流体装置由聚二甲基硅氧烷（PDMS）制成，具有9个通道，每个通道的深度为40μm，宽度范围为30μm至200 urn。使用注射泵推动直径为15μm的荧光微球的水通过微通道，流速范围为0.5μl/ min至10 ul / min。以25帧/秒的速度捕获视频。使用跟踪荧光微球运动的算法来计算微通道中微球的速度。该成像系统能够测量范围从0.2毫米/秒到10毫米/秒的粒子速度。淋巴管中感兴趣的流速范围为1 mm / sec至10 mm / sec；因此，我们的成像系统足以测量模拟淋巴流的体模中的粒子速度。

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