Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues

机译：组织中胶原结构的前向与后向偏振分辨SHG成像

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摘要

Second harmonic generation (SHG) is a powerful technique to observe fibrillar collagen without any staining and with a good contrast. More information about the molecular structure of collagen fibrils in tissues and their 3D distribution can be gained with polarization-resolved SHG imaging. Nevertheless, strong focusing is required for effective imaging and light propagation in tissues is complex and not thoroughly understood yet, preventing accurate and reproducible measurements. Theoretical analysis, vectorial numerical simulations and experiments were implemented to understand how the SHG signal builds up and how geometrical parameters affect polarization-resolved measurements in homogeneous collagen-rich tissues.

机译：二次谐波生成（SHG）是一种强大的技术，可观察到纤维状胶原而无任何染色且具有良好的对比度。通过偏振分辨SHG成像可以获得有关组织中胶原蛋白纤维的分子结构及其3D分布的更多信息。然而，有效的成像需要强力聚焦，并且光在组织中的传播是复杂的，并且尚未得到充分的理解，从而阻碍了准确和可重复的测量。进行了理论分析，矢量数值模拟和实验，以了解SHG信号如何建立以及几何参数如何影响均质胶原蛋白丰富组织中的偏振分辨测量。

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来源
《Multiphoton microscopy in the biomedical sciences XVI》|2016年|971218.1-971218.7|共7页
会议地点 San Francisco CA(US)
作者
Claire Teulon; Ivan Gusachenko; Gaeel Latour; Marie-Claire Schanne-Klein;
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作者单位

LOB, Ecole Polytechnique, CNRS, Inserm, Universite Paris-Saclay, 91128 Palaiseau, France;

LOB, Ecole Polytechnique, CNRS, Inserm, Universite Paris-Saclay, 91128 Palaiseau, France,Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna-son, Kunigami-gun Okinawa, Japan 904-0495;

IMNC, Univ. Paris-Sud, Univ. Paris-Diderot, CNRS, Universite Paris-Saclay, 91405 Orsay, France;

LOB, Ecole Polytechnique, CNRS, Inserm, Universite Paris-Saclay, 91128 Palaiseau, France;

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原文格式 PDF
正文语种 eng
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关键词
Multiphoton microscopy; SHG imaging; collagen; polarimetry; vectorial numerical calculations; cornea; tendon;

机译：多光子显微镜； SHG成像；胶原;旋光法向量数值计算；角膜;肌腱;

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专利

1. Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage [J] . Houle Marie-Andree, Couture Charles-Andre, Bancelin Stephane, Journal of Biophotonics . 2015,第11a12期

机译：分析向前和向后的第二谐波生成图像，以探测骨骼和软骨内胶原的纳米级结构
2. Effect of refractive index mismatch on forward-to-backward ratios in SHG imaging [J] . van der Kolk Jarno N., Bancelin Stephane, Kioulos Charalambos, Optics Letters . 2018,第20期

机译：折射率不匹配对SHG成像中向后比率的影响
3. Quantitative assessment of forward and backward second harmonic three dimensional images of collagen Type I matrix remodeling in a stimulated cellular environment [J] . Abraham Thomas, Kayra Damian, McManus Bruce, Journal of Structural Biology . 2012,第1期

机译：在刺激的细胞环境中对I型胶原基质重构的前向和后向二次谐波三维图像的定量评估
4. Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues [C] . Claire Teulon, Ivan Gusachenko, Gaeel Latour, Conference on multiphoton microscopy in the biomedical sciences XVI . 2016

机译：转发与组织中胶原结构的向后偏振分辨的SHG成像
5. The influence of looking backward versus forward on goal pursuit. [D] . Koo, Minjung. 2009

机译：向后看与向前看对目标追求的影响。
6. Quantitative assessment of forward and backward second harmonic three dimensional images of collagen Type I matrix remodeling in a stimulated cellular environment [O] . T Abraham, D Kayra, B McManus, -1

机译：向前和向后的二次谐波三维Ⅰ型胶原基质的重塑图像的定量评价刺激的细胞环境
7. Polarization-Resolved SHG towards Collagen Imaging [O] . Peres Chiara, DAutilia Francesca, Lanzanò Luca, 2014

机译：偏振分辨SHG走向胶原成像

Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues

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