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首页> 外文会议>Nanoscale imaging, sensing, and actuation for biomedical applications XI >Improving of Enzyme Immunoassay for Detection and Quantification of the Target Molecules Using Silver Nanoparticles
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Improving of Enzyme Immunoassay for Detection and Quantification of the Target Molecules Using Silver Nanoparticles

机译：改进的酶免疫测定法，用于检测和定量使用银纳米粒子的目标分子。

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Modern routine enzyme immunoassays for detection and quantification of biomolecules have several disadvantages such as high cost, insufficient sensitivity, complexity and long-term execution. The surface plasmon resonance of silver nanoparticles gives reasons of creating new in the basis of simple, highly sensitive and low cost colorimetric assays that can be applied to the detection of small molecules, DNA, proteins and pollutants. The main aim of the study was the improving of enzyme immunoassay for detection and quantification of the target molecules using silver nanoparticles. For this purpose we developed method for synthesis of silver nanoparticles with hyaluronic acid and studied possibility of use these nanoparticles in direct determination of target molecules concentration (in particular proteins) and for improving of enzyme immunoassay. As model we used conventional enzyme immunoassays for determination of progesterone and estradiol concentration. We obtained the possibility to produce silver nanoparticles with hyaluronan homogeneous in size between 10 and 12 nm, soluble and stable in water during long term of storage using modified procedure of silver nanoparticles synthesis. New method allows to obtain silver nanoparticles with strong optical properties at the higher concentrations - 60-90 μg/ml with the peak of absorbance at the wavelength 400 nm. Therefore surface plasmon resonance of silver nanoparticles with hyaluronan and ultraviolet-visible spectroscopy provide an opportunity for rapid determination of target molecules concentration (especial protein). We used silver nanoparticles as enzyme carriers and signal enhancers. Our preliminary data show that silver nanoparticles increased absorbance of samples that allows improving upper limit of determination of estradiol and progesterone concentration.
机译：用于检测和定量生物分子的现代常规酶免疫测定法具有多个缺点，例如成本高，灵敏度不足，复杂性和长期执行。银纳米粒子的表面等离振子共振提供了在简单，高度灵敏和低成本的比色测定法基础上创建新方法的原因，这些方法可用于检测小分子，DNA，蛋白质和污染物。该研究的主要目的是改进用于使用银纳米颗粒检测和定量靶分子的酶免疫法。为此，我们开发了用透明质酸合成银纳米颗粒的方法，并研究了将这些纳米颗粒用于直接确定目标分子浓度（特别是蛋白质）和改善酶免疫测定的可能性。作为模型，我们使用常规酶免疫法测定孕酮和雌二醇的浓度。我们获得了使用修饰的银纳米颗粒合成程序生产透明质酸银纳米颗粒的可能性，该纳米颗粒具有在10至12 nm之间大小均一的透明质酸，在长期存储过程中可溶于水并在水中稳定。新方法可以在60-90μg/ ml的较高浓度下获得具有强光学性能的银纳米颗粒，并且在400 nm波长处具有吸收峰。因此，透明质酸和紫外-可见光谱法检测银纳米颗粒的表面等离子体共振为快速测定目标分子浓度（特别是蛋白质）提供了机会。我们使用银纳米颗粒作为酶载体和信号增强剂。我们的初步数据表明，纳米银颗粒提高了样品的吸收率，从而提高了测定雌二醇和孕酮浓度的上限。

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