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Imaging microscopy by phase-contrast engine: Retardation-Modulated differential interference contrast microscope

机译：相衬引擎成像显微镜：延迟调制微分干涉对比显微镜

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摘要

In the field of biology and medicine, observation object of the microscope has been changing from the thin specimen to the thick living tissue. Furthermore, observation of the internal structure of a living tissue is also desired by low invasion. However, the real structure of a phase object with three-dimensional distribution such as a living tissue is difficult to observe, because of the influence of the phase distribution before and behind of observation position. We enabled observation of the internal structure of living tissue without stain, by adding a new function to reduce the influence of phase distribution to our Retardation-Modulated differential interference contrast (RM-DIC) microscope system.

机译：在生物学和医学领域中，显微镜的观察对象已经从薄样本变为厚活组织。此外，还期望通过低侵入来观察活组织的内部结构。但是，由于观察位置前后的相位分布的影响，难以观察到具有活体组织等三维分布的相位对象的真实结构。通过添加新功能以减少相位分布对我们的延迟调制微分干涉对比（RM-DIC）显微镜系统的影响，我们能够观察没有染色的活组织的内部结构。

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来源
《Optical design and testing VI》|2014年|92720A.1-92720A.5|共5页
会议地点 Beijing(CN)
作者
Hiroshi Ishiwata; Masahide Itoh;
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作者单位

Institute of Applied Physics, University of Tsukuba, 1-1-1 Tenohdai, Tsukuba, Ibaraki, Japan 305-8577;

Institute of Applied Physics, University of Tsukuba, 1-1-1 Tenohdai, Tsukuba, Ibaraki, Japan 305-8577;

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原文格式 PDF
正文语种 eng
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关键词
DIC microscope; 3D measurement; microstructure; phase object; deconvolution; deblurring;

机译：DIC显微镜3D测量微观结构相对象去卷积去模糊;

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专利

1. A new analysis for extending the measurement range of the retardation-modulated differential interference contrast (RM-DIC) microscope [J] . Hiroshi Ishiwata, Masahide Itoh, Toyohiko Yatagai Optics Communications: A Journal Devoted to the Rapid Publication of Short Contributions in the Field of Optics and Interaction of Light with Matter . 2008,第6期

机译：扩展延迟调制微分干涉对比（RM-DIC）显微镜的测量范围的新分析
2. Confocal scanning light microscopy of the Escherichia coli nucleoid: comparison with phase-contrast and electron microscope images. [J] . J A Valkenburg, C L Woldringh, G J Brakenhoff, Journal of bacteriology . 1985,第2期

机译：大肠埃希氏菌核苷的共聚焦扫描光学显微镜：与相差和电子显微镜图像的比较。
3. Widening the range of high-precision quantitative measurement in retardation-modulated differential interference microscope [J] . Ishiwata H., Nagai H., Naka T., Optical review . 2010,第3期

机译：拓宽了延迟调制微分干涉显微镜中的高精度定量测量范围
4. Quantitative phase analysis in retardation-modulated differential interference contrast (RM-DIC) microscope [C] . Hiroshi Ishiwata, Olympus Optical Co., Tokyo, Interferometry '99: Techniques and Technologies . 1999

机译：延迟调制微分干涉对比（RM-DIC）显微镜中的定量相分析
5. Motion analysis and visualization of biological structures imaged via Nomarski differential interference contrast light microscopy. [D] . Feineigle, Patricia Ann. 1996

机译：通过Nomarski微分干涉对比光学显微镜成像的生物结构的运动分析和可视化。
6. Confocal scanning light microscopy of the Escherichia coli nucleoid: comparison with phase-contrast and electron microscope images. [O] . J A Valkenburg, C L Woldringh, G J Brakenhoff, 1985

机译：大肠埃希氏菌核苷的共聚焦扫描光学显微镜：与相差和电子显微镜图像的比较。
7. A new analysis for extending the measurement range of the retardation-modulated differential interference contrast (RM-DIC) microscope [O] . Ishiwata Hiroshi, Itoh Masahide, Yatagai Toyohiko 2008

机译：扩展延迟调制微分干涉对比（RM-DIC）显微镜的测量范围的新分析
8. Image contrast and phase modulated light methods in polarization and inteference microscopy. Instruction manual for the PAR EPM-1 photoelectric polarizing and interference microscope [R] . Allen, R. D. 1964

机译：偏振和干涉显微镜中的图像对比和相位调制光方法。 paR Epm-1光电偏振和干涉显微镜使用说明书

Imaging microscopy by phase-contrast engine: Retardation-Modulated differential interference contrast microscope

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