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首页> 外文会议>Optical methods for tumor treatment and detection: mechanisms and techniques in photodynamic therapy XXV >Photodynamic inactivation of contaminated blood with Staphylococcus aureus
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Photodynamic inactivation of contaminated blood with Staphylococcus aureus

机译：金黄色葡萄球菌对血液的光动力灭活

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The presence of bacteria in the bloodstream can trigger a serious systemic inflammation and lead to sepsis that cause septic shock and death. Studies have shown an increase in the incidence of sepsis over the years and it is mainly due to the increased resistance of microorganisms to antibiotics, since these drugs are still sold and used improperly. The bacterial contamination of blood is also a risk to blood transfusions. Thus, bacteria inactivation in blood is being studied in order to increase the security of the blood supply. The purpose of this study was to decontaminate the blood using the photodynamic inactivation (PDI). Human blood samples in the presence of Photogem were illuminated at an intensity of 30 mW/cm~2, and light doses of 10 and 15 J/cm~2. Blood counts were carried out for the quantitative evaluation and blood smears were prepared for qualitative and morphological evaluation by microscopy. The results showed normal viability values for the blood cells analyzed. The light doses showed minimal morphological changes in the membrane of red blood cells, but the irradiation in the presence of the photosensitizer caused hemolysis in red blood cells at the higher concentrations of the photosensitizer. Experiments with Staphylococcus aureus, one of the responsible of sepsis, showed 7 logs_(10) of photodynamic inactivation with 50 μg/mL and 15 J/cm~2 and 1 log_(10) of this microorganism in a co-culture with blood.
机译：血液中细菌的存在会引发严重的全身性炎症并导致败血症，从而导致败血性休克和死亡。研究表明，多年来脓毒症的发病率增加，这主要是由于微生物对抗生素的耐药性增加，因为这些药物仍未正确销售和使用。血液的细菌污染也有输血的危险。因此，为了提高血液供应的安全性，正在研究血液中细菌的失活。这项研究的目的是使用光动力灭活（PDI）对血液进行净化。在存在Photogem的情况下，以30 mW / cm〜2的强度以及10和15 J / cm〜2的光剂量照射人体血液样品。进行血细胞计数以进行定量评估，并通过显微镜检查对血涂片进行定性和形态评估。结果显示所分析血细胞的正常生存力值。光剂量在红细胞膜上显示出最小的形态变化，但是在光敏剂存在下的照射在较高浓度的光敏剂下引起红细胞溶血。金黄色葡萄球菌（败血症的原因之一）的实验显示，在与血液共培养的条件下，用50μg/ mL和15 J / cm〜2的光致微生物灭活了7 log_（10）和1 log_（10）。

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