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Measurement of Ciliary Beat Frequency Using Ultra-High Resolution Optical Coherence Tomography

机译:使用超高分辨率光学相干断层扫描技术测量纤毛搏动频率

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摘要

Ciliated epithelial cells populate up to 80% of the surface area of the human airway and are responsible for mucociliary transport, which is the key protective mechanism that provides the first line of defense in the respiratory tract. Cilia beat in a rhythmic pattern and may be easily affected by allergens, pollutants, and pathogens, altering ciliary beat frequency (CBF) subsequently. Diseases including cystic fibrosis, chronic obstructive pulmonary disease, and primary ciliary dyskinesia may also decrease CBF. CBF is therefore a critical component of respiratory health. The current clinical method of measuring CBF is phase-contrast microscopy, which involves a tissue biopsy obtained via brushing of the nasal cavity. While this method is minimally invasive, the tissue sample must be oriented to display its profile view, making the visualization of a single layer of cilia challenging. In addition, the conventional method requires subjective analysis of CBF, e.g., manually counting by visual inspection. On the contrary, optical coherence tomography (OCT) has been used to study the retina in ophthalmology as well as vasculature in cardiology, and offers higher resolution than conventional computed tomography and magnetic resonance imaging. Based on this technology, our lab specifically developed an ultra-high resolution OCT system to image the microstructure of the ciliated epithelial cells. Doppler analysis was also performed to determine CBF. Lastly, we also developed a program that utilizes fast Fourier transform to determine CBF under phase-contrast microscopy, providing a more objective method compared to the current method.
机译:纤毛上皮细胞占人气道表面积的80%,负责粘膜纤毛运输,这是在呼吸道中提供第一道防线的关键保护机制。纤毛节律性跳动,很容易受到过敏原,污染物和病原体的影响,随后改变纤毛跳动频率(CBF)。囊性纤维化,慢性阻塞性肺疾病和原发性睫状运动障碍等疾病也可降低CBF。因此,CBF是呼吸系统健康的重要组成部分。当前测量CBF的临床方法是相差显微镜,该技术涉及通过鼻腔刷洗获得的组织活检。尽管此方法具有微创性,但必须对组织样本进行定向以显示其轮廓视图,从而使单层纤毛的可视化具有挑战性。另外,常规方法需要对CBF进行主观分析,例如通过目测手动计数。相反,光学相干断层扫描(OCT)已被用于研究眼科视网膜以及心脏病的脉管系统,并且比传统的计算机断层扫描和磁共振成像具有更高的分辨率。基于此技术,我们的实验室专门开发了超高分辨率的OCT系统,以对纤毛上皮细胞的微观结构进行成像。还进行了多普勒分析以确定CBF。最后,我们还开发了一个程序,该程序利用快速傅立叶变换在相差显微镜下确定CBF,与当前方法相比,它提供了一种更加客观的方法。

著录项

  • 来源
    《Photonic therapeutics and diagnostics XII》|2016年|968926.1-968926.7|共7页
  • 会议地点 San Francisco CA(US)
  • 作者单位

    Beckman Laser Institute Medical Clinic, University of California - Irvine, Irvine, CA, USA;

    Beckman Laser Institute Medical Clinic, University of California - Irvine, Irvine, CA, USA,Department of Biomedical Engineering, University of California - Irvine, Irvine, CA, USA;

    Beckman Laser Institute Medical Clinic, University of California - Irvine, Irvine, CA, USA;

    School of Medicine, University of California - Irvine, Irvine, CA, USA;

    Department of Otolaryngology - Head and Neck Surgery, School of Medicine, University of California - Irvine, Irvine, CA, USA;

    Beckman Laser Institute Medical Clinic, University of California - Irvine, Irvine, CA, USA,Department of Biomedical Engineering, University of California - Irvine, Irvine, CA, USA;

    Beckman Laser Institute Medical Clinic, University of California - Irvine, Irvine, CA, USA,Department of Otolaryngology - Head and Neck Surgery, School of Medicine, University of California - Irvine, Irvine, CA, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ciliary Beat Frequency; Cilia Airway; Mucociliary Clearance; Ultra High Resolution Optical Coherence Tomography;

    机译:睫状跳动频率;纤毛航空公司;粘膜纤毛间隙;超高分辨率光学相干断层扫描;

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