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Battling Bacterial Biofilms with Gas Discharge Plasma

机译：与气体放电等离子体对抗细菌生物膜

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摘要

Most studies dealing with growth and physiology of bacteria have been carried out using free-living cells. However, most bacteria live in communities referred to as biofilms where cooperative interactions among their members make conventional methods of controlling microbial growth often ineffective. The use of gas discharge plasmas represents an alternative to traditional decontamination/ sterilization methods. We studied biofilms using two organisms, Chromobacterium violaceum and Pseudomonas aeruginosa. With the first organism we demonstrated almost complete loss of cell culturability after a 5-min plasma treatment. However, additional determinations showed that non-culturable cells were still alive after short exposure times. We have recently reported the effect of plasma on P. aeruginosa biofilms grown on borosilicate coupons. In this paper, we present results for plasma treatments of 1-, 3-, and 7-day old P. aeruginosa biofilms grown on polycarbonate or stainless-steel coupons. Results indicate nearly 100% of biofilm inactivation after 5 min of exposure with similar inactivation kinetics for 1-, 3-, and 7-day-old biofilms, and for both materials used. The inactivation kinetics is similar for both organisms, suggesting that the method is useful regardless of the type of biofilm. AFM images show changes in biofilm structure for various plasma exposure times.
机译：大多数涉及细菌生长和生理的研究都是使用自由细胞进行的。但是，大多数细菌生活在称为生物膜的社区中，在这些社区中，其成员之间的协作相互作用使得控制微生物生长的常规方法通常无效。气体放电等离子体的使用代表了传统去污/灭菌方法的替代方法。我们研究了使用两种生物体的生物膜，紫罗兰色杆菌和铜绿假单胞菌。对于第一种生物，我们证明在5分钟的血浆处理后，细胞的可培养性几乎完全丧失。但是，另外的测定表明不可培养的细胞在短时间暴露后仍然存活。我们最近报道了血浆对在硼硅酸盐试样上生长的铜绿假单胞菌生物膜的影响。在本文中，我们介绍了在聚碳酸酯或不锈钢试样上生长的1天，3天和7天大的铜绿假单胞菌生物膜的等离子体处理结果。结果表明，暴露1分钟，3天和7天的生物膜以及所用的两种材料，暴露5分钟后生物膜的失活率接近100％，具有相似的失活动力学。两种生物的失活动力学相似，这表明该方法无论生物膜的类型如何都是有用的。 AFM图像显示了在各种血浆暴露时间下生物膜结构的变化。

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  • 来源
  • 会议地点 Demanovska Dolina(SK)
  • 作者

    Anna Zelaya; Kurt Vandervoort; Graciela Brelles-Marino;

  • 作者单位

    Biological Sciences Department, California State Polytechnic University, 3801 W. Temple Ave., Pomona, CA 91768, USA;

    Physics Department, California State Polytechnic University, Pomona, CA, USA;

    Biological Sciences Department, California State Polytechnic University, Pomona, CA, USA Center for Research and Development of Industrial Fermentations, (CINDEFI, CCT LA PLATA-CONICET), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物工程学（生物技术）;
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