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首页> 外文会议>Plasmonics in biology and medicine XIII >Simultaneous detection of multiple biomarkers by means of SERS on polymer nanopillar - gold arrays
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Simultaneous detection of multiple biomarkers by means of SERS on polymer nanopillar - gold arrays

机译：通过SERS在聚合物纳米柱-金阵列上同时检测多种生物标记

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摘要

The detection of biomarkers by means of Surface Enhanced Raman Spectroscopy (SERS) is foreseen to became a very important tool in the clinical practice because of its excellent sensitivity and potential for the simultaneous detection of multiple biomarkers. In the present paper we describe how it was possible to build a sensor for the detection of genetic biomarkers involved in acute myeloid leukemia. The assay is based on the use of a specifically designed SERS substrate made of a 2D crystal structure of polymeric pillars embedded in a gold layer. This substrate is characterized by good enhancing properties coupled with an excellent homogeneity. The SERS substrate was conjugated with DNA probes complementary to a target sequence and used in a sandwich assay with gold nanoparticles labeled with a second DNA probe and a Raman reporter. The so developed assay allowed the detection of a leukemia biomarker (WTl gene) and an housekeeping gene with low picomolar sensitivity. At last, we optimized the assay in order to tackle one of the main limitations of SERS based assay: the loss of signal that is observed when the Raman spectra are collected in liquid. Combining a preferential functionalization on the polymeric pillars with a different height of the polymer pillars from the gold layer the assay demonstrated its effectiveness even when measured in buffer.
机译：预计通过表面增强拉曼光谱法（SERS）进行生物标志物的检测将成为临床实践中非常重要的工具，因为它具有出色的灵敏度和同时检测多种生物标志物的潜力。在本文中，我们描述了如何构建一种传感器来检测涉及急性髓性白血病的遗传生物标记。该测定基于使用特别设计的SERS基底，该基底由嵌入金层中的聚合物柱的2D晶体结构制成。该基材的特征在于良好的增强性能以及优异的均匀性。 SERS底物与与靶序列互补的DNA探针缀合，并用于夹心测定，其中金纳米颗粒用第二个DNA探针和拉曼报道分子标记。如此开发的测定法允许检测具有低皮摩尔敏感性的白血病生物标志物（WT1基因）和管家基因。最后，我们优化了检测方法，以解决基于SERS的检测方法的主要局限之一：当在液体中收集拉曼光谱时观察到的信号丢失。将聚合物柱上的优先官能化与距金层不同高度的聚合物柱相结合，即使在缓冲液中进行测定，该测定也证明了其有效性。

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  • 来源
    《Plasmonics in biology and medicine XIII》|2016年|972404.1-972404.8|共8页
  • 会议地点 San Francisco CA(US)
  • 作者

    Carlo Morasso; Silvia Picciolini; Dora Mehn; Paola Pellacani; Ana Frangolho; Gerardo Marchesini; Renzo Vanna; Alice Gualerzi; Marzia Bedoni; Franco Marabelli; Furio Gramatica;

  • 作者单位

    IRCCS 'S. Maria Nascente' Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy;

    IRCCS 'S. Maria Nascente' Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy;

    IRCCS 'S. Maria Nascente' Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy;

    Plasmore s.r.l., Via Deledda 4, Ranco (VA), Italy;

    Plasmore s.r.l., Via Deledda 4, Ranco (VA), Italy;

    BioSyPher, 5 Fowey Av., IG4 5JT, Ilford, United Kingdom;

    IRCCS 'S. Maria Nascente' Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy;

    IRCCS 'S. Maria Nascente' Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy;

    IRCCS 'S. Maria Nascente' Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy;

    University of Pavia, Via Bassi 6, Pavia, Italy;

    IRCCS 'S. Maria Nascente' Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    SERS; Raman; Biosensor; Biomarker Detection; Cancer; Nanoparticles; 2D array;

    机译：SERS;拉曼生物传感器生物标志物检测；癌症;纳米颗粒； 2D阵列;

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