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Reduction of phase volume error in off-axis quantitative phase microscopy using optimum phase-shift

机译:使用最佳相移减少离轴定量相显微镜中的相体积误差

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we present a method to reduce the inherent errors caused by band-pass filter in off-axis quantitative phase microscopy and propose the optimum condition that can minimize these errors. We found that phase information of a sample in frequency domain nonlinearly oscillates as a function of the phase-shift correspond to the sample and its medium and the phase information of a sample inside the band-pass filter can be maximized by a proper phase-shift. Through numerical simulations and actual experiments, we demonstrate that the error in phase volume measurement can be effectively reduced by the enhancement of phase signal inside band-pass region using an optimum amount of phase that can be controlled by either changing medium index or wavelength of illumination.
机译:我们提出了一种减少轴外定量相位显微镜中由带通滤波器引起的固有误差的方法,并提出了可以使这些误差最小化的最佳条件。我们发现,样本中的相位信息在频域中作为相移的函数非线性地振荡,对应于样本及其介质,并且通过适当的相移,可以最大化带通滤波器内部样本的相位信息。 。通过数值模拟和实际实验,我们证明,通过使用可通过改变介质折射率或照明波长来控制的最佳相位量来增强带通区域内的相位信号,可以有效地减少相位体积测量中的误差。

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