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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Lignosus rhinocerus (Cooke) Ryvarden-A New Potential Medicinal Mushroom that Can Stimulate Neurite Outgrowth at Low Concentrations of Extracts
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Lignosus rhinocerus (Cooke) Ryvarden-A New Potential Medicinal Mushroom that Can Stimulate Neurite Outgrowth at Low Concentrations of Extracts

机译：Lignosus rhinocerus（Cooke）Ryvarden-一种新的潜在药用蘑菇，可在低浓度提取物中刺激神经突生长

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Alzheimer's disease is characterized by the loss of neurons and synapses in the cerebral cortex and some subcortical regions. Research has shown that absence of nerve growth factor causes Alzheimer-like symptoms in the adult brain of mice. A well known medicinal mushroom, Hericium erinaceus was found to have active compound/s with NGF-like properties and able to cross blood-brain barrier. The aim of this study was to investigate a new potential mushroom that had been used for medicinal purposes by local community in Malaysia. Neurite outgrowth in cultured rat pheochromocytoma cells, PC-12 acts as an indication of regenerative potential. Differentiated cells with one thin extension at least double the length of the cell diameter were scored positive. Our results showed that maximum outgrowth was shown at 20 μg/mL (w/v) for both L. rhinocerus aqueous and ethanol extract whereas for H. erinaceus maximal outgrowth was shown at 50 (μg/mL (w/v) of aqueous extract and 70 μg/mL (w/v) of ethanol extract. Lignosus rhinocerus extracts showed maximal neurite outgrowth at lower concentrations compared to H. erinaceus extracts. This showed that L. rhinocerus extracts may contain compound/s that able to enhance neurite outgrowth activity at a lower concentration. MTT assay was performed to check the percentage of cell viability after treatment with L. rhinocerus extract. IC50 value of aqueous extract of L. rhinocerus was recorded at 445. 15 μg/mL (w/v) and R value was 0. 988 4 while IC50 value of ethanol extract of L. rhinocerus was 282. 15 μg/mL (w/v) and R value was 0.991 8. IC50 values showed that extracts tested had no cytotoxic effect at low concentrations. To our knowledge, limited work has been done on neurite outgrowth activity using L. rhinocerus. Based on this preliminary result, further development and investigations are needed to confirm the mechanism / pathway for the neurite outgrowth. To our knowledge, this is the first report on neurite outgrowth stimulation activity by Lignosus rhinocerus.
机译：阿尔茨海默氏病的特征是大脑皮层和某些皮质下区域神经元和突触的丧失。研究表明，神经生长因子的缺乏会在成年小鼠的大脑中引起类似阿兹海默症的症状。人们发现一种著名的药用蘑菇猴头猴具有具有类似于NGF的特性的活性化合物，并且能够穿过血脑屏障。这项研究的目的是调查一种新的潜在蘑菇，该蘑菇已被马来西亚当地社区用于医疗目的。在培养的大鼠嗜铬细胞瘤细胞中神经突向外生长，PC-12可作为再生潜力的指标。具有一个细延伸至少两倍于细胞直径长度的分化细胞被评为阳性。我们的结果表明，犀牛水提取物和乙醇提取物的最大增幅均显示为20μg/ mL（w / v），而麻黄藻的最大提取物显示为50（μg/ mL（w / v）的水提取物和70μg/ mL（w / v）的乙醇提取物，木犀属犀牛提取物在较低浓度下显示出最大的神经突向外生长，这比erinaceus提取物低，这表明犀牛提取物可能包含能够增强神经突生长的化合物。在较低浓度下进行MTT分析以检查犀牛提取物处理后细胞活力的百分比，记录犀牛水提取物的IC50值为445. 15μg/ mL（w / v）和R值为0. 988 4，而犀牛乙醇提取物的IC50值为282. 15μg/ mL（w / v），R值为0.9918。IC50值表明所测试的提取物在低浓度下无细胞毒性作用。知识，使用L对神经突生长活动所做的有限工作。犀牛。基于此初步结果，需要进一步的发展和研究以确认神经突生长的机制/途径。据我们所知，这是首次关于木犀的神经突增生活性的报道。

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