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首页> 外文期刊>Evidence-based complementary and alternative medicine: eCAM >Lignosus rhinocerus(Cooke) Ryvarden: A Medicinal Mushroom That Stimulates Neurite Outgrowth in PC-12 Cells
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Lignosus rhinocerus(Cooke) Ryvarden: A Medicinal Mushroom That Stimulates Neurite Outgrowth in PC-12 Cells

机译：Lignosus rhinocerus（Cooke）Ryvarden：刺激PC-12细胞中神经突生长的药用蘑菇。

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A national treasure mushroom,Lignosus rhinocerus, has been used to treat variety of ailments by local and indigenous communities in Malaysia. The aim of this study was to investigate the potential of the most valuable part ofL. rhinocerus, the sclerotium, on neurite outgrowth activity by using PC-12Adh cell line. Differentiated cells with one thin extension at least double the length of the cell diameter were scored positive. Our results showed that aqueous sclerotiumL. rhinocerusextract induced neurite outgrowths of 24.4% and 42.1% at 20 μg/mL (w/v) of aqueous extract alone and a combination of 20 μg/mL (w/v) aqueous extract and 30 ng/mL (w/v) of NGF, respectively. Combination of NGF and sclerotium extract had additive effects and enhanced neurite outgrowth. Neuronal differentiation was demonstrated by indirect immunofluorescence of neurofilament protein. Aqueous sclerotium extract contained neuroactive compounds that stimulated neurite outgrowthin vitro. To our knowledge this is the first report on neurite-stimulating activities ofL. rhinocerus.
机译：马来西亚的国宝蘑菇Lignosus rhinocerus已被用来治疗各种疾病。这项研究的目的是调查L最有价值的部分的潜力。通过使用PC-12Adh细胞系，犀牛菌核对神经突的生长具有活性。具有一个细延伸至少两倍于细胞直径长度的分化细胞被评为阳性。我们的结果表明，水菌核。单独以20μg/ mL（w / v）的水提取物和以20μμg/ mL（w / v）的水提取物与30μng/ mL（w / v）的水提取物的组合，犀牛诱发的神经突增生分别为24.4％和42.1％。 NGF，分别。 NGF和菌核提取物的组合具有累加作用并增强了神经突的长出。通过神经丝蛋白的间接免疫荧光证明了神经元的分化。菌核提取物含有神经活性化合物，可在体外刺激神经突向外生长。据我们所知，这是关于L的神经突刺激活性的第一份报告。犀牛。

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