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New cross-linking quinoline and quinolone based luminescent lanthanide probes for sensitive labeling

机译:用于灵敏标记的新型交联喹啉和喹诺酮基发光镧系元素探针

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摘要

New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of chromophores: 7-amino-4-trifluoromethyl-2(lH) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent spectroscopy. Some of the compounds displayed high brightness with Tb~(3+), Eu~(3+), and Dy~(3+). Obtained reactive lanthanide chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beacon-based DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell imaging.
机译:合成了在天线荧光团部分中含有硫醇基,胺基和点击反应基团的新型发光镧系螯合物。螯合物包括与两种生色团偶联的二亚乙基三胺五乙酸(DTPA):7-氨基-4-三氟甲基-2(1H)喹啉酮和7-氨基-4-三氟甲基-2-烷氧基喹啉。使用NMR,光吸收,稳态和时间分辨荧光光谱对合成的化合物进行表征。某些化合物的Tb〜(3 +),Eu〜(3+)和Dy〜(3+)具有较高的亮度。获得的反应性镧系元素螯合物可以很容易地附着在生物分子上。这些探针在基于分子信标的DNA杂交测定(亚皮摩尔摩尔检测极限),细菌蛋白质组标记和活细胞成像中显示出高性能。

著录项

  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者单位

    Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, 151 Tiernan Hall, University Heights, Newark, New Jersey 07102, USA,PHRI Center, New Jersey Medical School, Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, New Jersey 07103, USA;

    Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, 151 Tiernan Hall, University Heights, Newark, New Jersey 07102, USA,PHRI Center, New Jersey Medical School, Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, New Jersey 07103, USA;

    PHRI Center, New Jersey Medical School, Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, New Jersey 07103, USA,Enghelhardt Institute for Molecular Biology RAN Moscow, Vavilova 32, Russia;

    PHRI Center, New Jersey Medical School, Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, New Jersey 07103, USA;

    Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, 151 Tiernan Hall, University Heights, Newark, New Jersey 07102, USA;

    PHRI Center, New Jersey Medical School, Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, New Jersey 07103, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用物理学;
  • 关键词

    lanthanide ion probes; molecular beacons; time-resolved detection; click reaction;

    机译:镧系离子探针分子信标时间分辨检测;点击反应;

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