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首页> 外文会议>The 18th international drying symposium >PURIFICATION AND DRYING OF STEVIOL GLYCOSIDES FROM STEVIA REBAUDIANA BERTONI
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PURIFICATION AND DRYING OF STEVIOL GLYCOSIDES FROM STEVIA REBAUDIANA BERTONI

机译：甜叶菊中甜菊醇糖苷的纯化和干燥

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摘要

In the present study,the purification of Steviol glycosides from the leaves of S.rebaudiana was carried out using different physical separation techniques such as extraction and crystallization followed by vacuum drying.The extraction of the Steviol glycosides from the leaves of S.rebaudiana was carried out using hot water.The effect of Steviol glycosides to water ratio on extraction efficiency was studied.It was found that the Steviol glycosides to water ratio of 1:35 gave higher percentage recovery of Steviol glycosides.The extract clarification is an important stage as it imparts better visual quality to the final product and this was done using decolouring and flocculating agent such as calcium hydroxide followed by the bleaching treatment using sulphur dioxide.Subsequent to extract clarification,the techniques of evaporation and crystallization were effectively employed for further purification of Steviol glycosides with a recovery of 95％ Steviol glycosides of high purity and reasonably high percentage of rebaudioside A in the product.The process of crystallization was studied in terms of supersaturation (sweeter component).In order to get steviol glycosides crystals in the dry form,vacuum drying was carried out and it was found that steviol glycosides gives crystals in the range 30-70 μm after drying.A new method for HPLC analysis was also developed for the analysis of Steviol glycosides.
机译：本研究采用不同的物理分离技术，如提取，结晶，真空干燥等方法，从沙棘叶中提取甜菊醇糖苷，然后从沙枣叶中提取甜菊醇糖苷。研究了甜菊糖苷与水的比例对提取效率的影响，发现甜菊糖苷与水的比例为1:35时，甜菊糖苷的回收率更高，因此提纯是一个重要的阶段。赋予最终产品更好的视觉质量，使用脱色和絮凝剂（例如氢氧化钙），然后使用二氧化硫进行漂白处理。在提取物澄清之后，有效地利用了蒸发和结晶技术进一步纯化了甜菊醇糖苷回收率高达95％的高纯度甜菊醇甜菊醇产品中的莱鲍迪甙A的百分含量非常高。就过饱和度（甜味剂成分）研究了结晶过程。为了得到干燥形式的甜菊醇糖苷晶体，进行了真空干燥，发现甜菊糖苷得到干燥后结晶范围为30-70μm。还开发了一种新的HPLC分析方法，用于分析甜菊醇糖苷。

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