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首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; Progress in Biomedical Optics and Imaging; vol.7 no.13 >Measuring the surface topography at focal adhesions using laser feedback interferometry
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Measuring the surface topography at focal adhesions using laser feedback interferometry

机译：使用激光反馈干涉仪测量粘着斑处的表面形貌

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摘要

Reflection interference contrast microscopy of living cells has not yet fully matured. One goal would be to temporally resolve the distance between the cell and the substratum at each point over the cell surface. We have combined phase shifting laser feedback interferometry with a high numerical aperture inverted microscope in order to determine the topography of the ventral surface of a cell. We have obtained a map of both the topography of a cell as well as its reflectivity. Our data demonstrate that interference microscopy can be adapted to yield a measure of the distance between the cell and the substratum. We have quantified the ventral surface topology at focal adhesions and we have shown that these changes are correlated with markers for a focal adhesion adaptor protein. The laser feedback interferometer was used to determine the ventral surface topography of fixed metastatic mammary adenocarcinoma cells. The ventral surface of the cell was scanned by moving the sample with a piezoelectric stage. The height of the ventral surface, as well as the reflectivity, were determined using phase shifting interferometry. An overlay of a fluorescence image with the interference data shows that the prominent dark regions of the interference image correlate with the location of the paxillin.
机译：活细胞的反射干涉对比显微镜还没有完全成熟。一个目标是在时间上解析细胞表面上每个点处的细胞与基质之间的距离。我们已将相移激光反馈干涉仪与高数值孔径倒置显微镜相结合，以确定细胞腹面的形貌。我们已经获得了细胞形貌及其反射率的图。我们的数据表明，干涉显微镜可以适用于测量细胞与基质之间的距离。我们已经量化了粘连的腹表面拓扑结构，并且我们已经表明，这些变化与粘连适配器蛋白的标记物相关。激光反馈干涉仪用于确定固定的转移性乳腺癌细胞的腹面形貌。通过用压电平台移动样品来扫描细胞的腹面。使用相移干涉术确定腹面的高度以及反射率。荧光图像与干涉数据的叠加表明，干涉图像的突出暗区与Paxillin的位置相关。

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