• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Molecular characterization of interactions between TMV replicase protein and auxin responsive proteins: Implications in disease development. .
【24h】

Molecular characterization of interactions between TMV replicase protein and auxin responsive proteins: Implications in disease development. .

机译：TMV复制酶蛋白和生长素应答蛋白之间相互作用的分子表征：对疾病发展的影响。 。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tobacco Mosaic Virus and Arabidopsis thaliana serve as ideal model systems to study the molecular aspects of virus-host interactions. Using this system, an interaction between the helicase domain within TMV replicase protein and an auxin responsive protein, IAA26 was identified. IAA26 is a member of the Aux/IAA family of transcription factors that function as repressors in signaling pathways controlled by the phytohormone auxin. Characterization of the interaction was carried out utilizing a helicase mutant defective in its interaction with IAA26 and by creating transgenic plants silenced for IAA26 expression. These studies suggest that the interaction was not essential for either viral replication or movement but promoted the development of disease symptoms. Cellular co-localization studies revealed that in TMV infected tissue, the nuclear localization and stability of IAA26 was compromised and the protein was relocalized to distinct cytoplasmic vesicles in association with the viral replicase. In keeping with its role as a transcription factor, the alterations in IAA26 function should lead to misregulation of downstream auxin responsive genes and this is supported by the fact that ∼30% of the Arabidopsis genes displaying transcriptional alterations to TMV could be linked to the auxin signaling pathway. Aux/IAA family members share significant sequence and functional homology, and an additional interaction screen identified two more Arabidopsis Aux/IAA proteins, IAA27 and IAA18 and a putative tomato Aux/IAA protein, LeIAA26 that could interact with TMV helicase. The nuclear localization of these three proteins was disrupted by TMV and alterations in LeIAA26 levels induced virus infection-like symptoms in tomato. Additionally, transgenic plants over-expressing a proteolysis resistant mutant of IAA26 showed abnormal developmental phenotype, the severity of which was abrogated during TMV infection which blocked nuclear accumulation of the protein. Taken together, these findings suggest that TMV induced disease symptoms can partially be explained by the ability of the virus to disrupt the functioning of interacting Aux/IAA proteins within susceptible hosts. The significance of such interactions is yet to be determined but it appears that they may be advantageous to the virus while infecting tissues that are in a developmentally static stage.
机译：烟草花叶病毒和拟南芥是研究病毒与宿主相互作用的分子方面的理想模型系统。使用该系统，鉴定了TMV复制酶蛋白内的解旋酶结构域与生长素应答蛋白IAA26之间的相互作用。 IAA26是Aux / IAA转录因子家族的成员，在植物激素生长素控制的信号通路中起阻遏作用。利用在与IAA26相互作​​用中有缺陷的解旋酶突变体，并通过沉默IAA26表达的转基因植物来进行相互作用的表征。这些研究表明，该相互作用对于病毒复制或运动不是必需的，但可促进疾病症状的发展。细胞共定位研究表明，在TMV感染的组织中，IAA26的核定位和稳定性受到损害，并且与病毒复制酶相关的蛋白质重新定位在不同的胞质囊泡中。为了保持其作为转录因子的作用，IAA26功能的改变应导致下游生长素应答基因的失调，这一事实得到了以下事实的支持：约30％的拟南芥显示TMV转录改变的基因可能与生长素有关信号通路。 Aux / IAA家族成员具有重要的序列和功能同源性，另外的相互作用筛选还鉴定出了另外两种拟南芥Aux / IAA蛋白IAA27和IAA18，以及一种可能与TMV解旋酶相互作用的推定番茄Aux / IAA蛋白LeIAA26。这三种蛋白质的核定位被TMV破坏，LeIAA26水平的改变引起番茄中病毒感染样症状。另外，过表达IAA26蛋白水解抗性突变体的转基因植物表现出异常的发育表型，其严重性在TMV感染期间被消除，从而阻止了蛋白质的核积累。综上所述，这些发现表明，TMV诱发的疾病症状可以部分通过病毒破坏易感宿主内相互作用的Aux / IAA蛋白功能的能力来解释。这种相互作用的重要性尚待确定，但似乎它们在感染处于发育静止期的组织时可能对病毒有利。

著录项

  • 作者

    Padmanabhan, Meenu Sreedevi.;

  • 作者单位

    University of Maryland, College Park.;

  • 授予单位 University of Maryland, College Park.;
  • 学科 Agriculture Plant Pathology.; Biology Virology.; Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 229 p.
  • 总页数 229
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;植物学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号