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首页> 外文学位 >Ethylene and carbon dioxide inhibit aflatoxin B1 biosynthesis in Aspergillus parasiticus grown on peanuts .
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Ethylene and carbon dioxide inhibit aflatoxin B1 biosynthesis in Aspergillus parasiticus grown on peanuts .

机译：乙烯和二氧化碳抑制花生上寄生曲霉中黄曲霉毒素B1的合成。

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摘要

Production of Aflatoxin B1 by Aspergillus parasiticus is inhibited when the fungus is grown on raw peanuts under exposure to different concentrations of ethylene and/or CO2. Ethylene, a natural plant growth hormone produced during fruit ripening, is food-safe and relatively inexpensive to generate in sufficient quantities for treatment of stored plant materials. Our objective was to determine if ethylene demonstrated potential for use in a modified storage atmosphere for reduction of aflatoxin production by A. parasiticus on stored crops.; Peanuts were used as a model crop. Conidiospores of Aspergillus parasiticus D8D3 were inoculated on peanuts contained in a Petri dish. Petri dishes were placed in a growth chamber and continually flushed with air containing variable concentrations of CO2 and/or ethylene. The growth chamber was incubated at 30°C in the dark for 5 days unless otherwise noted. Aflatoxin in peanuts was quantified by ELISA (Enzyme Linked Immunosorbent Assay) and Thin Layer Chromatography (TLC).; Ethylene inhibited aflatoxin production and the levels of inhibition depended on the concentration used. The greatest effect of ethylene and CO 2 in reducing aflatoxin accumulation was about 80% and 85% reduction, respectively.
机译：当真菌在未加工的花生上暴露于不同浓度的乙烯和/或二氧化碳下生长时，寄生曲霉产生的黄曲霉毒素B1被抑制。乙烯是水果成熟过程中产生的天然植物生长激素，对食品安全且相对便宜，可以产生足够的量用于处理储存的植物材料。我们的目标是确定乙烯是否显示出在改良的贮藏气氛中有潜力用于减少贮藏农作物中的寄生寄生曲霉产生黄曲霉毒素。花生被用作模型作物。将寄生米曲霉D8D3的分生孢子接种在培养皿中所含的花生上。将培养皿放置在生长室中，并连续用含有不同浓度的CO2和/或乙烯的空气冲洗。除非另有说明，否则将生长室在黑暗中于30°C孵育5天。花生中的黄曲霉毒素通过ELISA（酶联免疫吸附测定）和薄层色谱法（TLC）进行定量。乙烯抑制黄曲霉毒素的产生，抑制水平取决于所用浓度。乙烯和CO 2减少黄曲霉毒素积累的最大作用分别为减少约80％和85％。

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  • 作者

    Gunterus, Agnesia.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Agriculture Food Science and Technology.; Agriculture Plant Pathology.
  • 学位 M.S.
  • 年度 2005
  • 页码 89 p.
  • 总页数 89
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农产品收获、加工及贮藏;植物病理学;
  • 关键词

  • 入库时间 2022-08-17 11:42:19

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