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The influence of supplemental vitamin E and zinc on the response of mouse macrophages to lipopolysaccharide.

机译:补充维生素E和锌对小鼠巨噬细胞对脂多糖反应的影响。

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摘要

This study, conducted to determine the synergistic effect vitamin E and zinc supplementation has on the mouse macrophage's release of inflammatory mediators in response to an immune challenge, used the RAW 264.7 mouse macrophage cell line supplemented with combinations of 0 and 50 muM vitamin E and zinc. Lipopolysaccharide from 055:B5 E. coli was used at concentrations of 0, 0.1 and 1.0 mug/mL media to stimulate this release.; Nitric oxide production was decreased by 50 muM zinc/50 muM vitamin E supplemented and 0 muM zinc/50 muM vitamin E supplemented mediums, showing possible beneficial effects of vitamin E supplementation. No differences in the amount of prostaglandin-E2, tumour necrosis factor-alpha, or interleukin-10 due to vitamin E and zinc supplementation were found, although trends were shown. Inflammatory response mediators have been linked to inflammatory diseases; therefore, further studies are needed to determine if vitamin E and zinc supplementation could have synergistic effects.
机译:这项研究旨在确定补充维生素E和锌对小鼠巨噬细胞响应免疫挑战释放的炎性介质的协同作用,该研究使用了RAW 264.7小鼠巨噬细胞系,并添加了0和50μM维生素E和锌。使用来自055:B5大肠杆菌的脂多糖以0、0.1和1.0马克杯/毫升培养基的浓度刺激该释放。补充50μM锌/ 50μM维生素E和添加0μM锌/ 50μM维生素E的培养基会减少一氧化氮的产生,表明补充维生素E可能具有有益的作用。尽管显示了趋势,但未发现由于维生素E和锌的补充而导致的前列腺素E2,肿瘤坏死因子α或白介素10的量存在差异。炎症反应介质与炎症性疾病有关。因此,需要进一步研究以确定补充维生素E和锌是否可能具有协同作用。

著录项

  • 作者

    Bell, Mary Rachel.;

  • 作者单位

    Oklahoma State University.;

  • 授予单位 Oklahoma State University.;
  • 学科 Health Sciences Nutrition.
  • 学位 M.S.
  • 年度 2005
  • 页码 105 p.
  • 总页数 105
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 预防医学、卫生学;
  • 关键词

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