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首页> 外文学位 >Functionalization of gold and nanocrystalline diamond atomic force microscope tips for single molecule force spectroscopy.
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Functionalization of gold and nanocrystalline diamond atomic force microscope tips for single molecule force spectroscopy.

机译：金和纳米晶金刚石原子力显微镜尖端的功能化，用于单分子力光谱学。

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摘要

The atomic force microscope (AFM) has fueled interest in nanotechnology because of its ability to image surfaces at the nanometer level and act as a molecular force sensor. Functionalization of the surface of an AFM tip surface in a stable, controlled manner expands the capabilities of the AFM and enables additional applications in the fields of single molecule force spectroscopy and nanolithography. Two AFM tip functionalizations are described: the assembly of tripodal molecular tips onto gold AFM tips and the photochemical attachment of terminal alkenes to nanocrystalline diamond (NCD) AFM tips.;Two separate tripodal molecules with different linker lengths and a monopodal molecule terminated with biotin were synthesized to attach to a gold AFM tip for single molecule force spectroscopy. The immobilization of these molecules was examined by contact angle measurements, spectroscopic ellipsometry, infrared, and near edge x-ray absorption fine structure (NEXAFS) spectroscopy. All three molecules displayed rupture forces that agreed with previously reported values for the biotin--avidin rupture. The tripodal molecular tip displayed narrower distribution in their force histograms than the monopodal molecular tip. The performance of the tripodal molecular tip was compared to the monopodal molecular tip in single molecule force spectroscopy studies. Over repeated measurements, the distribution of forces for the monopodal molecular tip shifted to lower forces, whereas the distribution for the tripodal molecular tip remained constant throughout. Loading rate dependence and control experiments further indicated that the rupture forces of the tripod molecular tips were specific to the biotin--NeutrAvidin interaction.;The second functionalization method used the photochemical attachment of undecylenic acid to NCD AFM tips. The photochemical attachment of undecylenic acid to hydrogen-terminated NCD wafer surfaces was investigated by contact angle measurements, x-ray photoelectron, infrared, and NEXAFS spectroscopy. The same photochemical method was used to attach molecules to NCD AFM tips. Selective fluorescent labeling and adhesion measurements indicated that the functionalization of undecylenic acid was successful. Studies towards applying NCD AFM tips for nanolithography on a polymer surface are also described.
机译：原子力显微镜（AFM）引起了人们对纳米技术的兴趣，因为它能够在纳米级成像表面并充当分子力传感器。以稳定，受控的方式对AFM尖端表面的功能进行功能化，扩展了AFM的功能，并使其能够在单分子力谱和纳米光刻领域中得到更多应用。描述了两个AFM尖端功能化：将三脚架分子尖端组装到金AFM尖端上，以及末端烯烃与纳米晶金刚石（NCD）AFM尖端的光化学连接。合成后可附着在金AFM尖端上，以进行单分子力光谱分析这些分子的固定化通过接触角测量，椭圆偏振光谱，红外和近边缘X射线吸收精细结构（NEXAFS）光谱进行了检查。这三个分子都显示出与先前报道的生物素-亲和素断裂值一致的断裂力。三脚架分子的尖端在力直方图中的分布比单足类分子的尖端窄。在单分子力谱研究中，将三脚架分子尖端的性能与单足分子尖端进行了比较。经过反复的测量，单足分子尖端的力分布转移到较低的力，而三脚架分子尖端的力分布始终保持恒定。加载速率依赖性和对照实验进一步表明，三脚架分子尖端的断裂力是生物素-NeutrAvidin相互作用所特有的。第二种功能化方法是将十一碳烯酸与NCD AFM尖端进行光化学连接。通过接触角测量，X射线光电子，红外和NEXAFS光谱研究了十一碳烯酸对氢封端的NCD晶片表面的光化学附着。使用相同的光化学方法将分子连接到NCD AFM尖端。选择性荧光标记和粘附力测量表明十一碳烯酸的功能化成功。还描述了将NCD AFM尖端用于在聚合物表面进行纳米平版印刷的研究。

著录项

  • 作者

    Drew, Michael E.;

  • 作者单位

    University of Pennsylvania.;

  • 授予单位 University of Pennsylvania.;
  • 学科 Chemistry Analytical.;Nanoscience.;Chemistry Physical.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 343 p.
  • 总页数 343
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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