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首页> 外文学位 >Transport and Uptake of Anthocyanins in Gastric Tissue and Their Effect on the Gastric Inflammatory Response: Developing an in vitro Model Using the NCI-N87 Gastric Cell Line.
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Transport and Uptake of Anthocyanins in Gastric Tissue and Their Effect on the Gastric Inflammatory Response: Developing an in vitro Model Using the NCI-N87 Gastric Cell Line.

机译：花青素在胃组织中的运输和摄取及其对胃炎症反应的影响：使用NCI-N87胃细胞系开发体外模型。

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摘要

Anthocyanins are polyphenolic pigment compounds that are ubiquitous in the plant kingdom, imparting color to many fruits commonly consumed by humans. Anthocyanins have been researched for their many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. In order to maximize the benefits of anthocyanin intake for health (including for the development of functional foods), the uptake, metabolism, and bioavailability of anthocyanins throughout the gastrointestinal tract must be elucidated. Animal and human studies have shown that anthocyanin glycosides appear in plasma very rapidly after oral ingestion, suggesting a possible role of the stomach on the in vivo fate of anthocyanins. The effects of the gastric environment and chemical structure on the transport and uptake of anthocyanins in the stomach have not been well characterized. Cell culture models can be useful tools for investigating these behaviors; however the environment must be kept at physiological pH (7.4), not ideal for the study of the acidic environment of the stomach. The NCI-N87 cell line is an acid-stable model of the gastric epithelium, and has successfully been used to study drug metabolism in the stomach. We investigated the NCI-N87 cell line, seeded on 0.4 mum pore inserts, as a model to study the uptake and metabolism of anthocyanins in the stomach.;The NCI-N87 cell line formed a coherent monolayer that was stable over a period of 18-32 days post-confluence. The pH levels of apical chambers were adjusted to pH 3.0, 5.0, or 7.4, with a basolateral pH of 7.4, with minimal effects on monolayer integrity. A slight initial drop in monolayer integrity at pH 3.0 was quickly recovered by 120 min treatment at 37°C. The mucus layer generated by the cells trapped a small amount of anthocyanins, but was easily removed by washing and did not appear to have significant effects on the analysis of cellular uptake.;Transport and uptake of anthocyanins from chokeberry extract by NCI-N87 cells was dependent on treatment time, initial anthocyanin concentration, and apical pH. Overall anthocyanin recovery was significantly higher at apical pH 3.0 as compared with pH 5.0 and 7.4. As expected, over time and with increasing initial concentrations, the basolateral concentration of anthocyanins increased. The rate of anthocyanin transport increased over time, suggesting that the transport was not by passive diffusion alone. Generally, an apical pH of 3.0 showed the highest amount of transport, and pH 5.0 showed the lowest amount. There was also an observed effect of anthocyanin structure on transport, both for anthocyanins from chokeberry (significantly higher transport of cy-3-arabinoside and lower transport of cy-3-galactoside) and from red grape (higher transport of cyanidin-3-glu and lower transport of malvidin-3-glu). Finally, anthocyanins from chokeberry were shown to significantly inhibit increased IL-8 production in response to the IL-1beta induced inflammation of NCI-N87 cells. This suggests a potential anti-inflammatory activity of anthocyanins in the stomach, at least with respect to this acute inflammation model.;We have shown with this work that the NCI-N87 cell line was a useful model to study the behavior of anthocyanins in the stomach. The transport and uptake of anthocyanins in this model were dependent upon initial concentration, time, apical pH, and sometimes, chemical structure. This work is further evidence that the stomach may play a significant role in the in vivo fate of anthocyanins.
机译：花青素是植物界中普遍存在的多酚类色素化合物，可为人类通常食用的许多水果赋予颜色。花色苷已被研究出许多潜在的健康益处，包括抗氧化剂，抗炎，抗癌和心脏保护活性。为了最大程度地摄取花青素对健康（包括开发功能性食品）的益处，必须阐明整个胃肠道中花青素的吸收，代谢和生物利用度。动物和人体研究表明，口服摄入花青素苷后，血浆中很快就会出现花青素，这表明胃对花青素的体内命运可能具有作用。胃环境和化学结构对花色苷在胃中的运输和吸收的影响尚未得到很好的表征。细胞培养模型可能是研究这些行为的有用工具。但是，必须将环境保持在生理pH值（7.4）上，这对于研究胃的酸性环境而言并不理想。 NCI-N87细胞系是胃上皮的酸稳定模型，已成功用于研究胃中的药物代谢。我们研究了NCI-N87细胞系，该细胞系接种在0.4毫米的孔插入物上，作为研究胃中花色苷摄取和代谢的模型。; NCI-N87细胞系形成了一个连贯的单层，该单层在18年的时间内稳定汇合后-32天。将顶室的pH值调节至pH 3.0、5.0或7.4，基底外侧pH值为7.4，对单层完整性的影响最小。通过在37°C下处理120分钟，很快恢复了pH 3.0下单层完整性的轻微初始下降。细胞产生的粘液层捕获了少量的花色苷，但很容易通过洗涤去除，似乎对细胞摄取的分析没有显着影响。NCI-N87细胞从苦莓提取物中花色苷的转运和摄取为取决于治疗时间，初始花青素浓度和顶端pH。与pH 5.0和7.4相比，在顶端pH 3.0时花色苷的总回收率显着更高。正如预期的那样，随着时间的推移，随着初始浓度的增加，花青素的基底外侧浓度增加。花青素的转运速率随时间增加，这表明该转运不是单独通过被动扩散进行的。通常，顶端pH值3.0显示最高的运输量，而pH 5.0则显示最低的运输量。还观察到花青素结构对运输的影响，无论是来自苦莓的花青素（cy-3-阿拉伯糖苷的较高运输和cy-3-半乳糖苷的较低运输）还是来自红葡萄（花青素-3-glu的较高运输）并降低Malvidin-3-glu的运输）。最后，显示来自苦莓的花色苷可显着抑制IL-1β诱导的NCI-N87细胞炎症反应，从而增加IL-8的产生。这表明至少在这种急性炎症模型中，花色苷在胃中具有潜在的抗炎活性。我们已经通过这项工作表明，NCI-N87细胞系是研究花色苷在胃中行为的有用模型。胃。在此模型中，花色苷的转运和摄取取决于初始浓度，时间，顶端pH值，有时还取决于化学结构。这项工作进一步证明了胃可能在花色苷的体内命运中起重要作用。

著录项

  • 作者

    Atnip, Allison.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Food science.
  • 学位 Ph.D.
  • 年度 2014
  • 页码 144 p.
  • 总页数 144
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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