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Determination of the mechanism(s) by which direct-fed microbials control Escherichia coli O157:H7 in cattle.

机译:确定直接饲喂微生物控制牛中大肠杆菌O157:H7的机制。

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摘要

Scope and Method of Study. The purpose of this experiment was to investigate the possible mechanisms responsible for the success of the direct-fed microbials, Lactobacillus acidophilus and Propionibacterium freudenreichii (included in and marketed as BovamineRTM), in controlling Escherichia coli O157:H7 in cattle. In order to do this an E. coli O157:H7 challenge was conducted with ten steers. Five were fed the direct-fed microbials and five were fed the control treatment, lactose (direct-fed microbial carrier). Immunoglobulins, blood chemistry, complete blood counts, performance measures, and carriage of E. coli O157:H7 were measured throughout the trial.;Findings and Conclusions. An increased level of serum IgA was observed in the direct-fed microbial group post-challenge. The direct-fed microbial group also showed an increased amount of blood granulocytes and granulocytes as a percentage of white blood cells post-challenge. The direct-fed microbial group had a higher post-challenge increase in total lymphocytes while the control group had higher lymphocytes as a percentage of white blood cells post-challenge. The control group had higher dry matter intakes. Both groups exhibited similar levels of all measurements prior to the challenge.
机译:研究范围和方法。本实验的目的是研究可能直接控制微生物,嗜酸乳杆菌和弗氏丙酸杆菌(包括和以BovamineRTM出售)成功控制牛大肠杆菌E157:H7的机制。为了做到这一点,用十头牛进行了大肠杆菌O157:H7攻击。五只被喂食了直接饲喂的微生物,五只被喂给了对照治疗乳糖(直接喂食的微生物载体)。在整个试验过程中,均对免疫球蛋白,血液化学,全血细胞计数,性能指标以及大肠杆菌O157:H7的运输情况进行了测量。结果与结论。挑战后,直接饲喂微生物组的血清IgA水平升高。直接进食的微生物组还显示出攻击后白细胞的百分比增加了血液粒细胞和粒细胞的数量。直接饲喂微生物组的攻击后总淋巴细胞增多,而对照组的淋巴细胞在攻击后白细胞中所占的百分比更高。对照组的干物质摄入量较高。挑战之前,两组的所有测量均显示相似的水平。

著录项

  • 作者

    Guillen, Lacey Michele.;

  • 作者单位

    Oklahoma State University.;

  • 授予单位 Oklahoma State University.;
  • 学科 Agriculture Food Science and Technology.;Agriculture Animal Culture and Nutrition.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 136 p.
  • 总页数 136
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农产品收获、加工及贮藏;饲料;
  • 关键词

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