The effects of different explant types, different hormones and their concentration combinations as well as dark culture time on the regeneration of grape ' Manicure Finger' ( Vitis vinifera) were studied.The results showed that adventitious buds could regenerate from the isolated leaves, petioles and stem segnents; among them the regenerated rate of adventitious buds from the isolated petioles was maximum.TDZ enhanced the regeneration of adventitious buds from petioles better than 6 - BA.IBA was better than NAA and 2,4 - D in inducing the regeneration of adventitious buds from petioles.Petiole explants displayed the highest regenerated rate after 3 weeks' dark culture.Among all the media prepared, the optimum media for the regeneration of adventitious buds from leaves, petioles and stem segments were MS + 1.0 mg/L TDZ + 0.1 mg/L IBA, MS + 1.0 mg/L TDZ +0.1 mg/L IBA, MS + 1.0 mg/L TDZ + 0.2 mg/L IBA, respectively; their regenerated rates were 60.71％, 93.10％ and 47.37％, respectively; their propagation coefficients were 5.65, 5.91 and 5.00 separately.The regenerated adventitious buds were induced to take roots on 3/4 MS +0.35 mg/L IBA medium, then the full plantlets were cultivated for 35 ～ 50 d, and their survival rate reached 90％ after being trained and transplanted to the greenhouse.%以关人指葡萄为试材,研究了不同外植体类型、激素浓度配比以及暗培养时间等对器官再生的影响.结果表明:叶片、叶柄、茎段外植体均可再生出不定芽,其中叶柄的再生率最高;TDZ对美人指葡萄叶柄再生效果比6-BA好IBA诱导美人指葡萄叶柄不定芽再生率高于NAA和2,4-D;暗培养3周时,美人指葡萄叶柄的再生率最高.叶片再生的最适培养基为:MS+1.0 ng/L TDZ+0.1 mg/L IBA,再生率为60.71%,增殖系数为5.65;叶柄再生的最适培养基为:MS+1.0mg/LTDZ+0.1 mg/L IBA,再生率为93.10%,增殖系数为5.91;茎段再生的最适培养基为:MS+1.0 mg/L TDZ+0.2 mg/L IBA,再生率为47.37%,增殖系数为5.00.将再生不定芽置于3/4MS+0.35 mg/LIBA培养基上诱导生根,完整植株培养35～50d后炼苗移栽,成活率可达90%.
展开▼
