目的:建立高效液相色谱法同时测定人参皂苷Rb1、Rc、Rd、Rg3、CK和Rh2的方法.方法:采用ODSC18(4.6 mm×150 mm)色谱柱,流动相乙腈-0.05%磷酸水,梯度洗脱,流速1 Ml/min,检测波长203 nm,柱温35 ℃.结果:人参皂苷Rb1、Rc、Rd、Rg3、CK和Rh2分离效果良好,线性关系良好,相对标准偏差和回收率符合2010年版中华人民共和国药典要求.结论:本方法简便、准确、稳定、重现性好,可用于上述人参皂苷的含量测定.%Objective:To determine the content of ginsenoside Rb1, Rc, Rd, Rg3, CK and Rh2 simultaneously by HPLC. Methods:The samples were separated on an ODS C18(4.6 mm × 150 mm) column using acetonitrile-0. 05 ％ phosphoric acid solution as the mobile phase, with the gradient elution at a flow of 1.0 mL/min and 35 ℃ column temperature. The wavelength of the detector was set at 203 nm. Results: A good separation effect can be reached for ginsenoside Rb1, Rc, Rd, Rg3, CK and Rh2 which showed a good linear relationship between the peak area and the concentration of standard samples. The relative standard deviation (RSD) is good to meet the standard of pharmacopoeia of PRC. Conclusion:The method is simple, accurate, with a good reproducibility. It can be applied to qualitative and quantitative analysis of ginsenoside Rb1, Rc, Rd, Rg3, CK and Rh2.
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