Objective To investigate potiential functions of LMO4 in regulating proliferation and differentiation of acute promyelocytic leukemia cells ( NB4 ) . Methods The expression of LMO4 in the acute promyelocytic leuke-mia cell line (NB4) was detected using qRT-PCR and Western blot, respectively. Depending on lentivirus trans-duction, NB4 cells with overexpression of LMO4 were established. The MTT assay was performed to detect prolifer-ation;NB4 cells were induced by all trans retinoic acid ( ATRA) to differentiate into mature granulocytes, which could be detected by using NBT test or flow cytometry for analysis of CD11b+ cells. Results Western blot analysis showed that LMO4 ectopically expressed in NB4 cells, and expression level of LMO4 decreased during differentia-tion induced by ATRA. After transduced by lentivirus containing human LMO4 cDNA, the NB4 cell line with LMO4 overexpression was obtained according to detection of LMO4 expression using qRT-PCR and Western blot a-nalysis. Compared to the control cells, the MTT assay showed LMO4 overexpression in NB4 cell could improve cells proliferation; meanwhile, both NBT tests and flow cytometry analysis indicated that LMO4 overexpression could also increase NB4 differentiation efficiency depending on ATRA induction. Conclusion Ectopic expression of LMO4 in NB4 cells promotes cell proliferation, and increases differentiation potency of NB4 cells due to being a-vailable of sensitiveness to ATRA.%目的 探讨LMO4 对急性早幼粒白血病细胞系NB4细胞增殖和分化的影响. 方法 利用 qRT-PCR和 Western blot法检测 NB4 中 LMO4 表达;利用慢病毒介导 LMO4 的cDNA感染NB4 细胞后,建立过量表达 LMO4 的 NB4 细胞株;采用MTT法检测细胞增殖;使用全反式维甲酸( ATRA)诱导NB4细胞分化0 ~48 h,瑞氏染色观察分化的细胞特性;流式细胞术分析NB4 细胞分化后产生CD11b阳性的细胞比例. 结果 qRT-PCR 和 Western blot 结果均提示 NB4中LMO4表达增加;ATRA诱导NB4分化时,LMO4的表达降低;MTT结果证实过量表达LMO4促进NB4增殖;瑞氏染色和流式细胞术结果均显示,过量表达LMO4能够促进ATRA诱导NB4细胞分化. 结论 LMO4参与调节NB4细胞的增殖,并能增加NB4细胞对ATRA的敏感性,促进其分化.
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