以两个转TaEBP基因小麦株系及相应受体对照品种为材料,采用盆栽控水法研究了水分胁迫对转基因小麦拔节期抗旱相关生理生化指标的影响,并结合隶属函数法对转基因株系的抗旱性进行了综合评价.结果显示:随着水分胁迫加剧,各参试品种(系)的相对含水量、叶绿素含量、光合速率、气孔导度呈下降趋势,转基因小麦下降幅度较小;胞间CO2、丙二醛含量、质膜相对透性呈上升趋势,受体对照品种上升幅度更大;转基因小麦的脯氨酸含量显著高于对照品种.应用隶属函数法综合评价结果表明,转TaEBP基因小麦株系G221、G258比其受体对照具有较高的抗旱能力.%Using two transgenic wheat strains with TaEBP gene and corresponding recurrent parent as materials , a pot experiment was conducted under different water stress to compare droughtnesistance of different varieties by studying the effect of physiological and biochemical indexes on drought resistance of transgenic wheat during jointing stage , and then to make a comprehensive evaluation of droughinesistance with subordination function method . The results showed that with the increase of water stress , leaf relative water content, chlorophyll content, photosynthetic rate and stomatal conductance showed downtrend , and the transgenic wheat declined more slowly ; while intercellular CO2 , MDA content, plasma membrane permeability showed uptrend , but the corresponding contrast rose more steeply . The proline content of transgenic wheat was significantly higher than contrast varieties . Based on the comprehensive evaluation we found that transgenic wheat strains G221 and G258 with TaEBP gene have higher droughtnesistant capability than the corresponding contrast ones XN979 and HG35 .
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