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首页> 中文期刊> 《农业生物技术学报》 >不同水分胁迫下转W16小麦回交株系基因表达特性及其抗旱机制

不同水分胁迫下转W16小麦回交株系基因表达特性及其抗旱机制

         
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摘要

干旱应答元件结合蛋白(DREB)转录因子调控植物抗逆相关功能基因的表达,对提高植物抗逆境胁迫具有重要作用.本研究采用盆栽法在小麦(Tritium aestivum)拔节至抽穗期进行不同水分胁迫条件下,分析了野生型和转基因小麦回交株系转录因子基因W16的表达特点,并对其抗旱性的生理生化指标进行了测定.半定量RT-PCR结果表明:野生型在无干旱胁迫时,W16有微弱表达,随胁迫增强,表达上调,当表达量达到峰值后(12 h),随胁迫的加剧,表达量迅速下降,呈现“上升-峰值-下降”特点,而整个干旱胁迫过程中转基因植株W16在Ubiquitin启动子作用下表达恒定且表达水平较高;抗旱性生理生化机制分析表明:不同水分胁迫条件下,转基因株系的叶绿素含量、脯氨酸含量、可溶性蛋白含量、水分利用效率的变化均高于受体对照,尤其在重度干旱(SD)胁迫下的差异更显著.产量结果显示,在各水分条件下转基因株系产量都高于受体对照,其抗旱指数属于较强抗旱等级.研究结果说明了转基因小麦回交株系中W16的超表达,改良了转基因小麦抗旱性的生理生化特性,提高了转基因小麦的抗旱性能.为抗旱转基因小麦品种选育提供理论和方法参考.%The dehydration responsive element binding protein (DREB) transcription factors play important roles in improving plant tolerance of abiotic stresses through regulating the expressions of the stress-related genes. To study the characteristics of drought resistance of the transgenie plant, the expression patterns of transcription factor W16 were analyzed in wild type and backcross lines of transgenie wheat (Tritium aestivum) cultivated in pots under different water stress treatments from tillering to heading stages, and the physiological and biochemical indexes related to drought tolerance were investigated. Semi-quantative RT-PCR analysis revealed that the expression of W16 was weak under no water stress condition, and its expression was up-regulated along with the enhancement of the water stress and reached the peak (12 h), then its expression dropped rapidly at serious water stress, which displayed a curve of "up-max-down" in wild type. Whereas the expression of W16 under the control of Ubiquitin promoter was strongand stable in transgenic wheat during the whole water stresses. The analysis on the physiological and biochemical indexes related to drought tolerance indicated that the changes of cholorophyll content, proline content, soluble protein content and water use efficiency of the transgenic lines were significantly higher than that of wild type under different water stresses, having significantly more dissimilarities especially under severe drought stress (SD), exceeding maximally 8.48% in chlorophyll content, 124.72% in proline, 37.74% in soluble protein content and 32.75% in water use efficiency of the controls and being more sensitive to water in jointing stage. Soluble protein content increased under moderate drought stress (MD) and decreased under severe drought stress (SD). The yield of transgenic lines were higher than that of wild type under different water stress, increasing more significantly under drought stress. Respectively, the yields of the strains A29-15 and A31-8 increasd 4.00% and 6.22% under moderate drought stress (MD), and that of the strains A29-15 and A31-8 increasd 11.16% and 16.27% under serious water stress, and their drought tolerant index was in the category of strong drought tolerance. Our results showed that over expression of W16 improves physiological and biochemical characteristics related to drought, and improves drought resistance in the backcross lines of transgenic wheat, achieving the goal of increasing production.

著录项

  • 来源
    《农业生物技术学报》 |2012年第11期|1271-1281|共11页
  • 作者

    周永斌; 闵东红; 徐兆师; 陈明; 李连城; 马有志;

  • 作者单位

    西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,杨陵712100;

    西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,杨陵712100;

    中国农业科学院作物科学研究所/国家农作物基因资源和基因改良重大科学工程/农业部作物遗传改良与育种重点开放实验室,北京100081;

    中国农业科学院作物科学研究所/国家农作物基因资源和基因改良重大科学工程/农业部作物遗传改良与育种重点开放实验室,北京100081;

    中国农业科学院作物科学研究所/国家农作物基因资源和基因改良重大科学工程/农业部作物遗传改良与育种重点开放实验室,北京100081;

    中国农业科学院作物科学研究所/国家农作物基因资源和基因改良重大科学工程/农业部作物遗传改良与育种重点开放实验室,北京100081;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类
  • 关键词

    W16; 小麦; 表达特性; 生理生化指标; 抗旱性;

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