目的：研究细胞因子信号转导抑制因子1（SOCS1）基因沉默对人口腔癌细胞化疗药物敏感性的影响及相关机制。方法Western blotting 及实时荧光定量（PCR）验证 SOCS1干扰序列沉默人口腔表皮样癌细胞（KB 细胞）株中 SOCS1的表达水平；MTT比色法分析细胞对化疗药物敏感性的变化；流式细胞术检测细胞凋亡。结果将 SOCS1干扰片段转染 KB 细胞后，SOCS1 mRNA及蛋白表达水平均明显降低。沉默 SOCS1表达后，化疗药物5-氟尿嘧啶和长春新碱对 KB 细胞的半数抑制浓度（IC50）均显著变小（P ＜0．05），SOCS1沉默还可增加长春新碱诱导的细胞凋亡（P ＜0．05），SOCS1表达抑制后 KB 细胞核转录因子-κB（NF-κB）和半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）的蛋白表达水平明显降低，与对照组比较差异均有统计学意义（P ＜0．05）。结论SOCS1表达有效抑制后，人口腔癌细胞 KB 对化疗药物的敏感性增强，且促进化疗药物诱导的肿瘤细胞凋亡。%Objective To investigate the changes of chemotherapeutic drugs’sensitivity of human oral cancer after suppressor of cyto-kine signaling-1 (SOCS1)gene silencing.Methods Western blotting and real-time PCR were used to verify the downregulation ofSOCS1 human oral cancer cell line (KB)after transfection;and the change of chemotherapeutic drugs’sensitivity was detected by MTT assay;flow cytometry was used to detect the cell apoptosis rate.Results of RT-PCR and Western blotting showed that the expression levels of SOCS1 mRNA and protein were significantly decreased in small interfering SOCS1 (SOCS1-siRNA)transfected KB cells.After the silence of SOCS1,the median inhibitory concentration of chemotherapeutic drugs (5-fluorouracil and vincristine)for KB cells de-creased significantly (P <0.05).The cell apoptosis rate of vincristine for KB cells significantly increased after SOCS1 gene silencing (P <0.05).The NF-κB and Caspase-3 protein expression levels in the experiment group dropped significantly compared with that of control group (P <0.05).Conclusions Inhibition of SOCS1 gene expression of oral cancer KB cells enhances the sensitivity to che-motherapeutic drugs,and promotes the apoptosis of cancer cells induced by chemotherapeutic drugs.
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