建立饮料中维生素C含量的高效液相色谱检测方法.采用ODS C18(4.6mm×250mm,5μm)反相色谱柱,以0.1％草酸溶液为流动相,光电二级管阵列检测器,检测波长为267nm.线性范围为20 ～ 160μg/mL,相关系数为0.9983,方法的回收率为96.4％～100.2％,相对标准偏差为0.61％.该方法简便、分析速度快,能够满足果汁饮料中维生素C的检测要求.%In this work, HPLC has been used to determine the content of Vitamin C in beverage. ODS C18(4. 6mm x 250mm,5u,m) is used as reversed phase column. The 0. 1% oxalic acid solution is mobile phase. The wavelength of photodiode array detector is 267run. Its recovery-ratio is 96.4% ~ 100. 2% , the correlation coefficient is 0. 9963. The relative standard deviation was 0.61% . This HPLC method is simple and convenient,and is easy to control. This analysis method is fast-speed,high sensitivity,and be able to meet requirements of the Vitamin C in beverage industry.
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