Natural sweeteners extracted from natural plants, in safety and nutrition function far better than synthetic sweeteners. In this paper, liquid chroraatography - tandem mass spectrometry was used to determine mannitol, maltose , xylitol, sorbitolin of the residues in food, using selected ion monitoring for positive confirmation. Liquid sample is extracted with water by the ultrasonic processor, After centrifugation and volume, Solid samples or hemi - solid samples were dissolved constant volume of water, and then by HLB solid phase extraction cartridges, eluted sample solution was determined later for the determination and confirmation by LC - MS/MS using external standard method. The detection limits, linear ranges, and recoveries of this method, for mannitol, maltose, xylitol, sorbitolin, are 0. 10mg/kg、 0. 10 ~ 1000. 0mg/kg and 89. 0 ~94. 9% . This study established a method for determination of mannitol, maltose, xylitol, sorbitolin in foods, can quickly and sensitive, a simultaneous determination of many categories and natural sweeteners and its positive categories confirmation. Also is to break the foreign technical barriers to trade, enhance Chinas export products in the international market competition ability to provide strong guarantee.%天然甜味剂是从天然植物中提取出来的,在安全性和营养功能方面远好于人工合成的甜味剂.本文采用液相色谱串联质谱法测定了食品中甘露糖醇、麦芽糖、木糖醇、山梨糖醇的残留量,并采用选择离子检测进行阳性确证.液体试样用水超声提取,离心定容后,固体和半固体样品采用水溶解定容,再经固相萃取柱净化,样液经洗脱定容后,供液相色谱-质谱/质谱仪测定,外标法定量.本方法的最低检出限、线性范围和方法回收率分别为:0.10mg/kg、0.10～1000.0mg/kg和89.0～94.9％.本研究建立了食品中甘露糖醇、麦芽糖、木糖醇、山梨糖醇的检测方法,可以快速、灵敏、一次同时测定多个、多类别天然甜味剂及其阳性确证.也为打破国外技术性贸易壁垒、增强我国出口产品在国际市场竞争力提供了有力的保障.
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