Objective To study the levels of interleukin-37 (IL-37),interleukin-6 (IL-6),interleukin-18 (IL-18) and interferon-γ (IFN-γ) in serum and peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE),and to investigate the relationships of IL-37 expression with other cytokines in the patients.Methods From December 2012 to December 2015,32 patients with SLE in the Department of Rheumatology,the First Hospital of Jilin University were selected into SLE group,and 32 normal healthy people in the Medical Examination Center were selected into control group.The serum levels of IL-37,IL-6,IL-18 and IFN-γ and the expressions of IL-37 mRNA,IL-6 mRNA,IL-18 mRNA and IFN-γ mRNA in the peripheral blood mononuclear cells were detected in both group.Results The serum levels of IL-37,IL-6,IL-18 and IFN-γ in the SLE group were significantly higher than those in the control group (P ＜ 0.05).The serum IL-37 was positively correlated with serum IL-6,IL-18 and IFN-γin the SLE patients (P ＜ 0.05).Multivariate regression analysis showed that the levels of IL-6,IL-18 and IFN-γwere the influencing factors for serum IL-37 level (P ＜ 0.05).The expressions of IL-37 mRNA,IL-6 mRNA,IL-18 mRNA and IFN-γ mRNA in the peripheral blood mononuclear cells of the SLE group were higher than those of the control group (P ＜ 0.05).The IL-37 mRNA expression in the peripheral blood mononuclear cells was positively correlated with the expressions of IL-6 mRNA,IL-18 mRNA and IFN-γ mRNA in the SLE patients(P ＜ 0.05).Multivariate regression analysis showed that IL-18 mRNA,IL-6 mRNA and IFN-γ mRNA expression levels were the influencing factors for IL-37 mRNA expression in the peripheral blood mononuclear cells (P ＜ 0.05).Conclusions The newly discovered inflammatory factor IL-37 is involved in the pathogenesis of systemic lupus erythematosus,and is associated with IL-6,IL-18 and IFN-γ.%目的 对系统性红斑狼疮患者血清及外周血单个核细胞白细胞介素37(IL-37)、白细胞介素6(IL-6)、白细胞介素18(IL-18)和干扰素γ(IFN-γ)进行研究,探讨系统性红斑狼疮患者IL-37的表达及其与其他细胞因子的相关性.方法 选择2012年12月-2015年12月吉林大学第一医院风湿科系统性红斑狼疮患者32例和体检中心正常健康者32例作为研究对象,将32例系统性红斑狼疮患者作为系统性红斑狼疮组,将32例健康者作为对照组.检测两组患者血清IL-37、IL-6、IL-18和IFN-γ水平以及外周血单个核细胞IL-37 mRNA、IL-6 mRNA、IL-18 mRNA和IFN-γ mRNA的表达.结果 系统性红斑狼疮组患者血清IL-37、IL-6、IL-18和IFN-γ水平均高于对照组健康者(P＜0.05).系统性红斑狼疮患者血清IL-37与血清IL-6、IL-18、IFN-γ均呈正相关(P＜0.05).多元线性回归分析显示IL-6、IL-18、IFN-γ水平是血清IL-37水平的影响因素(均P ＜0.05).系统性红斑狼疮组IL-37 mRNA、IL-6 mRNA、IL-18 mRNA和IFN-γmRNA的表达均高于对照组(P＜0.05).系统性红斑狼疮患者外周血单个核细胞IL-37 mRNA与IL-6mRNA、IL-18 mRNA和IFN-γ mRNA均呈正相关(P＜0.05).多元线性回归分析显示IL-6 mRNA、IL-18mRNA、IFN-γ mRNA表达量是外周血单个核细胞IL-37 mRNA表达量的影响因素(均P＜0.05).结论 新发现的炎症因子IL-37参与系统性红斑狼疮的发病过程,与IL-6、IL-18和IFN-γ炎症因子具有相关性.
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