目的:探讨使用电化学发光免疫法(ECLIA)测定血清胰岛素及C-肽时标本溶血程度及溶血标本放置时间对测定结果的干扰.方法:将检测标本人为干预成5种不同溶血程度的溶血标本,置于室温20℃环境下,分为4个时间段(0 min、30 min、60 min、120 min)分别采用电化学发光免疫法测定溶血和非溶血标本的胰岛素及C-肽浓度并作分析.结果:标本溶血可使胰岛素浓度降低,胰岛素浓度测定与标本溶血程度及放置时间呈负相关；标本溶血对电化学发光免疫法测定C-肽无干扰.结论:测定胰岛素时要绝对避免溶血；在标本分析前质量控制不确定情况下C-肽释放水平曲线比胰岛素释放水平曲线在临床上更具可信性.%Objective: To approach specimen hemolysis degree and hemolytic specimen waiting period interfering with the testing results while using electrochemiluminescence immunoassay (ECLIA) to test serum insulin and C-peptide. Methods: We artificially interfered with the test specimen into 5 hemolysis specimens with different hemolysis degrees. And we placed them in indoor environment (20℃). Using ECLIA to test and analyze insulin and C-peptide degreernof hemolytic and non-hemolytic specimens separately during 4 different time quanta (0, 30, 60, 120 min). Results: Specimen hemolysis can lower insulin concentration. Insulin concentration testing and specimen hemolytic degree and waiting period are in negative correlation. Specimen hemolysis has no influence on using Electrochemiluminescence Immunoassay to test C-peptide. Conclusion: Definitely avoid hemolysis during insulin test. Under the circumstance of unsure quality control before specimen analysis, C-peptide releasing horizontal curve is more reliable in clinical practices than insulin releasing horizontal curve.
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