目的：评价染料木素的抗诱变性。方法培养L5178Y细胞,根据不同的处理方法进行分组,阳性诱变组[染料木素0.078、0.156、0.312、0.625μg/mL 4种剂量分别联合甲基磺酸甲酯(MMS)5μg/mL或丝裂霉素C(MMC)0.5μg/mL]、阳性诱变对照组(MMS 5μg/mL或MMC 0.5μg/mL)、溶剂对照组(0.5%二甲基亚砜)、阴性对照组(纯水)以及抗诱变阳性对照组(维生素C 100μg/mL+MMS 5μg/mL或MMC 0.5μg/mL)。通过L5178Y小鼠淋巴瘤细胞实验微孔板法,采用前处理(染料木素先处理细胞3 h,然后MMS或MMC处理3 h)、同时处理(染料木素与MMS或MMC同时处理细胞3 h)和后处理(MMS或MMC先处理细胞3 h,然后染料木素处理3 h)3种处理方式,测定染料木素各剂量组tk位点突变频率,评价染料木素对MMS或MMC诱变性的拮抗作用。结果在染料木素抗诱变性检测中,在前处理和后处理条件下,染料木素各剂量组tk位点总突变频率显著低于MMS或MMC阳性诱变对照组,差异有高度统计学意义(P<0.01)；在同时处理条件下,染料木素0.312、0.625μg/mL剂量组tk位点总突变频率显著低于MMS或MMC阳性诱变对照组,差异有高度统计学意义(P<0.01)。结论在3种处理方式下,染料木素能有效地抑制MMS和MMC的诱变作用,以前处理方式的抑制作用最强,有良好的开发利用前景。%Objective To evaluate the antimutagenic effect of Genistein. Methods L5178Y cells were cultivated, and grouped according to the different processing methods, the L5178Y cells were respectively exposed to the Genistein concentrations of 0.078, 0.156, 0.312, 0.625μg/mL with 5μg/mL methyl methanesulfonate (MMS)or 0.5 μg/mL mito-mycin C (MMC) (positive mutation group), 5 μg/mL MMS or 0.5 μg/mL MMC (positive mutation control group), 0.5%DMSO (solvent control group), water (negative control group), 5μg/mL MMS or 0.5μg/mL MMC and 100μg/mL VitC+5μg/mL MMS or 0.5μg/mL MMC (anti mutagenesis positive control group). The antimutagenic effect of genistein were determined by the mouse lymphoma Assay in L5178Y cells, in the evaluation of antimutagenic effect three kinds of treatment (pre-treatment, silmutaneous-treatment and post-treatment) were used to detect the antimutagenic effects of Genistein and puerarin against gene mutations induced by MMS and MMC. Determination of mutant frequency of tk lo-cus (TFT) was performed according to the microcell method. Results In pre-treatment and post-treatment, at all the tested concentrations the total mutation frequencies were lower than the MMS or MMC positive mutation control group the differences were statistically significant (P < 0.01). In simultaneous treatment, the total mutation frequencies at concentrations of 0.312, 0.625μg/mL were lower than MMS and MMC positive mutation control group, the differences were statistically significant (P﹤0.01). Conclusion Under the three kinds of treatment, Genistein shows a good role for pre-venting TK gene mutation induced by MMS or MMC, pre-treatment has the strongest inhibitory effect, had a good ap-plication and development prospect.
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