OBJECTIVE:To prepare Puerarin polymeric micelles and establish a method to determine its entrapment efficiency. METHODS:Puerarin polymeric micelles were prepared by film dispersion method. The polymeric micelles and free drug were sepa-rated by centrifugal-millipore filter filtration method. The entrapment efficiency of puerarin polymeric micelles was determined by HPLC. Diamonsil C18(2)column was used with 1% citric acid solution-methanol(65∶35)at the flow rate of 1 ml/min. The detec-tion wavelength was set at 250 nm,and column temperature was room temperature. RESULTS:The prepared polymeric micelles were spherical and spherical-like in shape with a mean particle size of 54.12 nm,polydispersity index of 0.122,Zeta potential of -13.60 mV;the linear range of puerarin was 2-10μg/ml(R2=0.999 4)with average recovery rate of 99.2%(RSD=0.9%,n=3). The re-covery rate of free drug was 95.3%(RSD=1.7%,n=3). The mean entrapment efficiency and drug-loading amount of puerarin were(35.5±2.12)% and(0.3±0.07)%,respectively(n=3). CONCLUSIONS:Film dispersion method is suitable for the prepara-tion of Puerarin polymeric micelles. Established method is convenient,accurate and reliable for the content and entrapment efficien-cy determination of Puerarin polymeric micelles.%目的：制备葛根素聚合物胶束并建立其包封率测定方法。方法：采用薄膜分散法制备葛根素聚合物胶束；以离心-微孔滤膜法分离胶束和游离药物，采用高效液相色谱法测定聚合物胶束包封率，色谱柱为Diamonsil C18（2），流动相为1％柠檬酸溶液-甲醇（65∶35），流速为1 ml/min，检测波长为250 nm，柱温为室温。结果：制备的聚合物胶束形态为规则的球形或类球形，平均粒径为54.12 nm，多分散系数为0.122，Zeta电位为－13.60 mV；葛根素检测质量浓度线性范围为2～10μg/ml（R2＝0.9994），方法回收率平均值为99.2％（RSD＝0.9％，n＝3），游离药物回收率为95.3％（RSD＝1.7％，n＝3）；葛根素胶束平均包封率为（35.5±2.12）％、载药量为（0.3±0.07）％（n＝3）。结论：薄膜分散法适用于制备葛根素聚合物胶束；所建立的葛根素含量及其包封率测定方法操作简单、准确、可靠。
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