目的：探讨6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3（ PFKFB3）在肝细胞癌（ HCC）组织中的表达及其与临床病理特征的关系。方法采用逆转录-聚合酶链反应（ RT-PCR）检测PFKFB3在42例HCC组织及其对应癌旁组织中的表达情况，并研究其与临床病理参数（性别、年龄、肿瘤直径、淋巴转移、静脉癌栓、治疗前甲胎蛋白、分化程度及肝内转移）间的关系，采用Kaplan-Meier法分析PFKFB3 mRNA表达对HCC患者生存的影响。结果 HCC和癌旁组织中PFKFB3 mRNA的阳性表达率分别为73�81％（31/42）和52�38％（22/42），相对表达量为1�075±0�171和0�877±0�451，HCC组织中 PFKFB3 mRNA的阳性表达率及相对表达量均高于癌旁组织（ P＜0�05）；PFKFB3 mRNA的表达与肿瘤TNM分期、分化程度、肝内转移及总生存期有关（ P＜0�05）。结论 PFKFB3在HCC的发生发展中发挥作用，且影响肝癌的分化、转移并提示肝癌患者预后不佳；可作为评估HCC恶性程度的生物学指标。%Objective To investigate the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase ( PFKFB3) in hepatocellular carcinoma( HCC) and its clinical significance. Methods The expressions of PFKFB3 mRNA of tissue sections from 42 HCC and paired adjacent tissues were detected by RT-PCR. Then the relationships between PFKFB3 expression and clinicopathological variables ( gender, age, tumor size, lymphatic metastasis, cancer embolus in vein, AFP level before treatment, differentiation and me-tastasis in liver) were analyzed. Meanwhile, the difference of overall survival of patients with different expression of PFKFB3 were stud-ied by survival analysis of Kaplan-meier. Results The positive expression rate and relative level of PFKFB3 in HCC tissue were 73�81% (31/42) and 1�075±0�171, higher than 52�38% (22/42) and 0�877±0�451 in adjacent tissues with significant difference ( P<0�05) . The PFKFB3 expression is related with TNM stage, degree of differentiation, intrahepatic metastasis and overall survival ( P<0�05) . Conclusion PFKFB3 were related to carcinogenesis, development and metastasis of HCC. The expression of PFKFB3 can influence the differentiation, metastasis andprognosis of HCC, indicating it may be used as biological indicators to evaluate the degree of malignancy of HCC.
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